دورية أكاديمية
Generating Retinal Injury Models in Xenopus Tadpoles
| العنوان: | Generating Retinal Injury Models in Xenopus Tadpoles |
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| المؤلفون: | Parain, Karine, Donval, Alicia, Chesneau, Albert, Lun, Jing Xian, Borday, Caroline, Perron, Muriel |
| المساهمون: | Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1940-087X. |
| بيانات النشر: | HAL CCSD JoVE |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | MESH: Animals, MESH: Xenopus laevis, MESH: Larva, MESH: Retina, Genetically Modified, MESH: Retinitis Pigmentosa, MESH: Mammals, [SDV]Life Sciences [q-bio] |
| الوصف: | Retinal neurodegenerative diseases are the leading causes of blindness. Among numerous therapeutic strategies being explored, stimulating self-repair recently emerged as particularly appealing. A cellular source of interest for retinal repair is the Müller glial cell, which harbors stem cell potential and an extraordinary regenerative capacity in anamniotes. This potential is, however, very limited in mammals. Studying the molecular mechanisms underlying retinal regeneration in animal models with regenerative capabilities should provide insights into how to unlock the latent ability of mammalian Müller cells to regenerate the retina. This is a key step for the development of therapeutic strategies in regenerative medicine. To this aim, we developed several retinal injury paradigms in Xenopus: a mechanical retinal injury, a transgenic line allowing for nitroreductase-mediated photoreceptor conditional ablation, a retinitis pigmentosa model based on CRISPR/Cas9-mediated rhodopsin knockout, and a cytotoxic model driven by intraocular CoCl2 injections. Highlighting their advantages and disadvantages, we describe here this series of protocols that generate various degenerative conditions and allow the study of retinal regeneration in Xenopus. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/37902314; hal-04308410; https://hal.science/hal-04308410Test; https://hal.science/hal-04308410/documentTest; https://hal.science/hal-04308410/file/jove-protocol-65771generating-retinal-injury-models.pdfTest; PUBMED: 37902314 |
| DOI: | 10.3791/65771 |
| الإتاحة: | https://doi.org/10.3791/65771Test https://hal.science/hal-04308410Test https://hal.science/hal-04308410/documentTest https://hal.science/hal-04308410/file/jove-protocol-65771generating-retinal-injury-models.pdfTest |
| حقوق: | http://hal.archives-ouvertes.fr/licences/copyrightTest/ |
| رقم الانضمام: | edsbas.F7F90F68 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3791/65771 |
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