دورية أكاديمية
Hexavalent chromium at low concentration alters Sertoli cell barrier and connexin 43 gap junction but not claudin-11 and N-cadherin in the rat seminiferous tubule culture model
| العنوان: | Hexavalent chromium at low concentration alters Sertoli cell barrier and connexin 43 gap junction but not claudin-11 and N-cadherin in the rat seminiferous tubule culture model |
|---|---|
| المؤلفون: | Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe |
| المساهمون: | Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Centre National de la Recherche Scientifique (CNRS), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE |
| المصدر: | ISSN: 0041-008X. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2013 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs] |
| الوصف: | International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003 |
| DOI: | 10.1016/j.taap.2013.01.016 |
| الإتاحة: | https://doi.org/10.1016/j.taap.2013.01.016Test https://hal.inrae.fr/hal-02651875Test |
| رقم الانضمام: | edsbas.F7BD6F41 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.taap.2013.01.016 |
|---|