دورية أكاديمية
Ex vivo detection and quantification of gold nanoparticles in human seminal and follicular fluids
| العنوان: | Ex vivo detection and quantification of gold nanoparticles in human seminal and follicular fluids |
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| المؤلفون: | Bitounis, Dimitrios, Klein, Jean-Philippe, Mery, Lionel, Maurice Edouard, El Merhie, Amira, Forest, Valérie, Boudard, Delphine, Pourchez, Jérémie, Cottier, Michèle |
| المساهمون: | Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Biologie de la Reproduction CHU Saint Etienne, Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon, F-69000 Lyon, France, Service de Cytologie - Histologie Rénale - Embryologie CHU Saint-Etienne (UF 6725 - UF 6726) |
| المصدر: | ISSN: 0003-2654. |
| بيانات النشر: | HAL CCSD Royal Society of Chemistry |
| سنة النشر: | 2018 |
| المجموعة: | Université Jean Monnet – Saint-Etienne: HAL |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], [SDV.IB]Life Sciences [q-bio]/Bioengineering |
| الوصف: | International audience ; Increasing consumption of engineered nanoparticles and occupational exposure to novel, ultrafine airborne particles during the last decades has coincided with deterioration of sperm parameters and delayed fecundity. In order to prevent possible adverse health effects and ensure a sustainable growth for the nanoparticle industry, the ability to investigate the nanosized, mineralogical load of human reproductive systems is becoming a real clinical need. Toward this goal, the current study proposes two methods for the detection and quantification of engineered nanoparticles in human follicular and seminal fluid, developed with the use of well-defined 60 nm Au particles. Despite the complexity of these biological fluids, simple physical and chemical treatments allow for the precise quantification of more than 50 and 70% wt of the spiked Au nanoparticles at low μg ml−1 levels in follicular and seminal fluids, respectively. The use of electron microscopy for the detailed observation of the detected analytes is also enabled. The proposed method is applied on a small patient cohort in order to demonstrate its clinical applicability by exploring the differences in the metal and particulate content between patients with normal and low sperm count. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01896549; https://hal.science/hal-01896549Test; https://hal.science/hal-01896549/documentTest; https://hal.science/hal-01896549/file/HAL_Bitounis%20et%20al_2018_Analyst.pdfTest |
| DOI: | 10.1039/c7an01641g |
| الإتاحة: | https://doi.org/10.1039/c7an01641gTest https://hal.science/hal-01896549Test https://hal.science/hal-01896549/documentTest https://hal.science/hal-01896549/file/HAL_Bitounis%20et%20al_2018_Analyst.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F7857EEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/c7an01641g |
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