كتاب
Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells
| العنوان: | Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells |
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| المؤلفون: | Linneberg-Agerholm, Madeleine, Brickman, Joshua Mark |
| المصدر: | Linneberg-Agerholm , M & Brickman , J M 2022 , Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells . in Methods in Molecular Biology . Humana Press , Methods in Molecular Biology , vol. 2416 , pp. 105-116 . https://doi.org/10.1007/978-1-0716-1908-7_8Test |
| بيانات النشر: | Humana Press |
| سنة النشر: | 2022 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| مصطلحات موضوعية: | Embryonic stem cells, Endoderm, Extra-embryonic, Human, Hypoblast, Naïve, Pluripotency, Primitive endoderm |
| الوصف: | In human, endoderm is induced in two waves, with the first being the extra-embryonic primitive endoderm (PrE), otherwise known as hypoblast, induced during blastocyst development, and the second being gastrulation-stage definitive endoderm (DE). The PrE gives rise to the primary and secondary yolk sac, and has supportive functions during pregnancy for nutrient provision, with descendants of this extra-embryonic lineage also playing a role in embryonic patterning. As in DE specification, we recently found that PrE could be induced in vitro by Wnt and Nodal-related signaling, but that the critical difference was in the pluripotent starting point for differentiation. Thus, blastocyst-like naïve human pluripotent stem cells retain the unique capacity to differentiate into PrE cultures, a cell type resembling the pre-implantation hypoblast. The PrE cells could then be expanded as stable naïve extra-embryonic endoderm (nEnd) cell lines, capable of indefinite self-renewal. Here, we describe detailed protocols to differentiate naïve pluripotent stem cells into PrE and then expand the cultures as nEnd, including descriptions of morphology, passaging technique, and troubleshooting. |
| نوع الوثيقة: | book part |
| اللغة: | English |
| DOI: | 10.1007/978-1-0716-1908-7_8 |
| الإتاحة: | https://doi.org/10.1007/978-1-0716-1908-7_8Test https://curis.ku.dk/portal/da/publications/differentiation-and-expansion-of-human-extraembryonic-endoderm-cell-lines-from-naive-pluripotent-stem-cellsTest(30910c10-f6a8-4016-ae5e-2684f2ee97f2).html |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F75275CE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-0716-1908-7_8 |
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