دورية أكاديمية
Recovery of normal DNA repair and mutagenesis in trichothiodystrophy cells after transduction of the XPD human gene.
| العنوان: | Recovery of normal DNA repair and mutagenesis in trichothiodystrophy cells after transduction of the XPD human gene. |
|---|---|
| المؤلفون: | Marionnet, C, Quilliet, X, Benoit, A, Armier, J, Sarasin, A, Stary, Anne |
| المساهمون: | Centre National de la Recherche Scientifique (CNRS), Institut de Recherches Scientifiques sur le Cancer Villejuif |
| المصدر: | ISSN: 0008-5472. |
| بيانات النشر: | HAL CCSD American Association for Cancer Research |
| سنة النشر: | 1996 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | MESH: Cell Line, Transformed, MESH: Colony-Forming Units Assay, MESH: Transfection, MESH: Ultraviolet Rays, MESH: DNA Helicases, MESH: Xeroderma Pigmentosum, MESH: Xeroderma Pigmentosum Group D Protein, MESH: DNA Repair, MESH: DNA-Binding Proteins, MESH: Fibroblasts, MESH: Genetic Complementation Test, MESH: Hair Diseases, MESH: Metabolism, Inborn Errors, MESH: Humans, MESH: Intellectual Disability, MESH: Mutagenesis, MESH: Photosensitivity Disorders, MESH: Protein Biosynthesis, MESH: Proteins, MESH: Recombinant Fusion Proteins, MESH: Sulfur, MESH: Transcription Factors, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics |
| الوصف: | International audience ; To determine whether expression of the XPD/ERCC2 repair gene in trichothiodystrophy (TTD) group D cells could restore mutagenesis characteristics of repair-proficient cells, we compared the UV mutagenesis of normal cells, TTD group D cells, and TTD group D cells retrovirally transduced by the wild-type XPD/ERCC2 gene (TTD + ERCC2 cells). We first verified the expression of the XPD protein, correction of UV cell survival, and DNA repair ability of TTD + ERCC2 cells. UV-induced mutations were studied using the pR2 shuttle vector. The addition of the XPD/ERCC2 gene in TTD cells led to a significant but partial decrease of mutation frequency compared with the parental TTD cells. Types of mutations of TTD + ERCC2 cells get closer to those observed in normal cells (ie., a reduction of multiple mutations). New hotspots appeared and some disappeared in the complemented line, suggesting that hotspot distribution is particular to each cell line and cannot be correlated with the repair status of the cells. In conclusion, the expression of the XPD/ERCC2 repair gene completely corrected UV hypersensitivity and almost all types of mutations of TTD group D cells, whereas hypermutagenesis was partially corrected. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/8968100; hal-03680168; https://hal.science/hal-03680168Test; https://hal.science/hal-03680168/documentTest; https://hal.science/hal-03680168/file/cr0560235450.pdfTest; PUBMED: 8968100 |
| الإتاحة: | https://hal.science/hal-03680168Test https://hal.science/hal-03680168/documentTest https://hal.science/hal-03680168/file/cr0560235450.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F7395F20 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |