دورية أكاديمية
Recombinant Human IFN alpha-2b Response Promotes Vaginal Epithelial Cells Defense against Candida albicans
| العنوان: | Recombinant Human IFN alpha-2b Response Promotes Vaginal Epithelial Cells Defense against Candida albicans |
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| المؤلفون: | Li, Ting, Niu, Xiaoxi, Zhang, Xu, Wang, Suxia, Liu, Zhaohui |
| المساهمون: | Liu, ZH (reprint author), Peking Univ, Dept Obstet & Gynecol, Hosp 1, Beijing, Peoples R China., Peking Univ, Dept Obstet & Gynecol, Hosp 1, Beijing, Peoples R China., Peking Univ, Lab Electron Microscopy, Hosp 1, Ultrastruct Pathol Ctr, Beijing, Peoples R China. |
| المصدر: | SCI |
| بيانات النشر: | FRONTIERS IN MICROBIOLOGY |
| سنة النشر: | 2017 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | vulvovaginal candidiasis, vaginal epithelial cells, Candida albicans, rhIFN alpha-2b, cytokines, INTERFERON-ALPHA, HOST-DEFENSE, FUNGAL-INFECTIONS, ANIMAL-MODELS, TH17 CELLS, IN-VITRO, PATHWAY, DISEASE, GAMMA, INTERLEUKIN-17 |
| الوصف: | Classical antifungal drugs have been subjected to restrictions due to drug toxicity, drug resistance, bioavailability, and detrimental drug interactions. Type I interferon (IFN) exerts direct distinct immunostimulatory or immunomodulatory actions; however, little is known regarding the anti-fungal reactions of vaginal epithelial cells (VECs) induced by the type I IFN response. Therefore, in the present study, we evaluated the cytotoxic activity, immunocompetent cytokine responses, and non-B IgG production of the VK2/E6E7 VEC line following recombinant human IFN alpha-2b (rhIFN alpha-2b) treatment in response to Candida albicans. When treated with rhIFN alpha-2b, the production of IL-2, IL-4, and IL-17 were significantly up-regulated compared to the infected control cells (P < 0.05). Our scanning electron microscopy results revealed that C. albicans can invade VECs by inducing both endocytosis and active penetration. RhIFN alpha-2b was able to transform the VECs into a thallus and stretched pattern, promoting the fusion of filopodia to form a lamellipodium and enhancing the mobility and the repair capacity of the VECs. In addition, rhIFN alpha-2b could effectively inhibit the adhesion, hyphal formation, and proliferation of C. albicans. Collectively, these responses restored the immune function of the infected VECs against C. albicans in vitro, providing a theoretical basis for this novel treatment strategy. ; National Natural Science Foundation of China [81571394] ; SCI(E) ; ARTICLE ; 8 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1664-302X |
| العلاقة: | FRONTIERS IN MICROBIOLOGY.2017,8.; 1908261; http://hdl.handle.net/20.500.11897/473937Test; WOS:000399533700003 |
| DOI: | 10.3389/fmicb.2017.00697 |
| الإتاحة: | https://doi.org/20.500.11897/473937Test https://doi.org/10.3389/fmicb.2017.00697Test https://hdl.handle.net/20.500.11897/473937Test |
| رقم الانضمام: | edsbas.F67CCEFA |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664302X |
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| DOI: | 10.3389/fmicb.2017.00697 |