التفاصيل البيبلوغرافية
| العنوان: |
A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection |
| المؤلفون: |
Schelde, Karen, Rosenjack, Julie, Sonneborn, Claire, Jafri, Anjum, Kavran, Michael, Brumbaugh, Sarah, Rietsch, Arne, Darrah, Rebecca J, Hodges, Craig A, Flask, Christopher A, Kelley, Thomas J, Drumm, Mitchell L |
| المساهمون: |
The Doris and Floyd Kimble Foundation and MPB Charitable Foundation, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland Digestive Diseases Research Core Center, Carlsbergfondet, Cleveland Clinical and Translational Science Collaborative, The Research Center for Children's Health, Cystic Fibrosis Foundation |
| المصدر: |
Laboratory Animals ; volume 57, issue 6, page 611-622 ; ISSN 0023-6772 1758-1117 |
| بيانات النشر: |
SAGE Publications |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
General Veterinary, Animal Science and Zoology |
| الوصف: |
The laboratory mouse is used extensively for human disease modeling and preclinical therapeutic testing for efficacy, biodistribution, and toxicity. The variety of murine models available, and the ability to create new ones, eclipses all other species, but the size of mice and their organs create challenges for many in vivo studies. For pulmonary research, improved methods to access murine airways and lungs, and track substances administered to them, would be desirable. A nonsurgical endoscopic system with a camera, effectively a bronchoscope, coupled with a cryoimaging fluorescence microscopy technique to view the lungs in 3D, is described here that allows visualization of the procedure, including the anatomical location at which substances are instilled and fluorescence detection of those substances. We have applied it to bacterial infection studies to characterize better and optimize a chronic lung infection murine model in which we instill bacteria-laden agarose beads into the airways and lungs to extend the duration of the infection and inflammation. The use of the endoscope as guidance for placing a catheter into the airways is simple and quick, requiring only momentary sedation, and reduces post-procedural mortality compared with our previous instillation method that includes a trans-tracheal surgery. The endoscopic method improves speed and precision of delivery while reducing the stress on animals and the number of animals generated and used for experiments. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1177/00236772231175553 |
| الإتاحة: |
https://doi.org/10.1177/00236772231175553Test |
| حقوق: |
https://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: |
edsbas.F63FA49D |
| قاعدة البيانات: |
BASE |
