التفاصيل البيبلوغرافية
| العنوان: |
Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase. |
| المساهمون: |
Bruck, Irina (authoraut), Kaplan, Daniel L (authoraut) |
| سنة النشر: |
2015 |
| المجموعة: |
Florida State University: DigiNole Commons |
| مصطلحات موضوعية: |
Blotting, Western, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, DNA Replication, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Humans, Minichromosome Maintenance Complex Component 2/genetics, Minichromosome Maintenance Complex Component 2/metabolism, Minichromosome Maintenance Proteins/genetics, Minichromosome Maintenance Proteins/metabolism, Mutation, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/growth & development, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Signal Transduction/genetics |
| الوصف: |
Dbf4-dependent kinase (DDK) phosphorylates minichromosome maintenance 2 (Mcm2) during S phase in yeast, and Sld3 recruits cell division cycle 45 (Cdc45) to minichromosome maintenance 2-7 (Mcm2-7). We show here DDK-phosphoryled Mcm2 preferentially interacts with Cdc45 in vivo, and that Sld3 stimulates DDK phosphorylation of Mcm2 by 11-fold. We identified a mutation of the replication initiation factor Sld3, Sld3-m16, that is specifically defective in stimulating DDK phosphorylation of Mcm2. Wild-type expression levels of sld3-m16 result in severe growth and DNA replication defects. Cells expressing sld3-m16 exhibit no detectable Mcm2 phosphorylation in vivo, reduced replication protein A-ChIP signal at an origin, and diminished Go, Ichi, Ni, and San association with Mcm2-7. Treslin, the human homolog of Sld3, stimulates human DDK phosphorylation of human Mcm2 by 15-fold. DDK phosphorylation of human Mcm2 decreases the affinity of Mcm5 for Mcm2, suggesting a potential mechanism for helicase ring opening. These data suggest a conserved mechanism for replication initiation: Sld3/Treslin coordinates Cdc45 recruitment to Mcm2-7 with DDK phosphorylation of Mcm2 during S phase. ; Keywords: DNA replication, Helicase, Initiation, Kinase, Phosphorylation ; Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568703Test. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
1 online resource; computer |
| اللغة: |
English |
| العلاقة: |
Proceedings of the National Academy of Sciences of the United States of America--1091-6490--1091-6490; fsu:523943; (IID) FSU_pmch_26305950; (DOI) 10.1073/pnas.1509608112; (PMCID) PMC4568703; (RID) 26305950; (EID) 26305950; (PII) 1509608112; https://diginole.lib.fsu.edu/islandora/object/fsu%3A523943/datastream/TN/view/Conserved%20mechanism%20for%20coordinating%20replication%20fork%20helicase%20assembly%20with%20phosphorylation%20of%20the%20helicase.jpgTest |
| الإتاحة: |
https://diginole.lib.fsu.edu/islandora/object/fsu%3A523943/datastream/TN/view/Conserved%20mechanism%20for%20coordinating%20replication%20fork%20helicase%20assembly%20with%20phosphorylation%20of%20the%20helicase.jpgTest |
| رقم الانضمام: |
edsbas.F6228AAB |
| قاعدة البيانات: |
BASE |
