التفاصيل البيبلوغرافية
| العنوان: |
Bacterial surface display of human lectins in Escherichia coli |
| المؤلفون: |
Vázquez‐Arias, Alba, Vázquez‐Iglesias, Lorena, Pérez‐Juste, Ignacio, Pérez‐Juste, Jorge, Pastoriza‐Santos, Isabel, Bodelon, Gustavo |
| المساهمون: |
Horizon 2020 Framework Programme, Ministerio de Ciencia e Innovación, Xunta de Galicia |
| المصدر: |
Microbial Biotechnology ; volume 17, issue 2 ; ISSN 1751-7915 1751-7915 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2024 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Lectin‐glycan interactions sustain fundamental biological processes involved in development and disease. Owing to their unique sugar‐binding properties, lectins have great potential in glycobiology and biomedicine. However, their relatively low affinities and broad specificities pose a significant challenge when used as analytical reagents. New approaches for expression and engineering of lectins are in demand to overcome current limitations. Herein, we report the application of bacterial display for the expression of human galectin‐3 and mannose‐binding lectin in Escherichia coli . The analysis of the cell surface expression and binding activity of the surface‐displayed lectins, including point and deletion mutants, in combination with molecular dynamics simulation, demonstrate the robustness and suitability of this approach. Furthermore, the display of functional mannose‐binding lectin in the bacterial surface proved the feasibility of this method for disulfide bond‐containing lectins. This work establishes for the first time bacterial display as an efficient means for the expression and engineering of human lectins, thereby increasing the available toolbox for glycobiology research. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/1751-7915.14409 |
| الإتاحة: |
https://doi.org/10.1111/1751-7915.14409Test |
| حقوق: |
http://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: |
edsbas.F5EB2A0A |
| قاعدة البيانات: |
BASE |
