دورية أكاديمية
Symmetric neural progenitor divisions require chromatin-mediated homologous recombination DNA repair by Ino80
| العنوان: | Symmetric neural progenitor divisions require chromatin-mediated homologous recombination DNA repair by Ino80 |
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| المؤلفون: | Keil, Jason M., Doyle, Daniel Z., Qalieh, Adel, Lam, Mandy M., Funk, Owen H., Qalieh, Yaman, Shi, Lei, Mohan, Nitesh, Sorel, Alice, Kwan, Kenneth Y. |
| المصدر: | Nature Communications ; volume 11, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | Chromatin regulates spatiotemporal gene expression during neurodevelopment, but it also mediates DNA damage repair essential to proliferating neural progenitor cells (NPCs). Here, we uncover molecularly dissociable roles for nucleosome remodeler Ino80 in chromatin-mediated transcriptional regulation and genome maintenance in corticogenesis. We find that conditional Ino80 deletion from cortical NPCs impairs DNA double-strand break (DSB) repair, triggering p53-dependent apoptosis and microcephaly. Using an in vivo DSB repair pathway assay, we find that Ino80 is selectively required for homologous recombination (HR) DNA repair, which is mechanistically distinct from Ino80 function in YY1-associated transcription. Unexpectedly, sensitivity to loss of Ino80 -mediated HR is dependent on NPC division mode: Ino80 deletion leads to unrepaired DNA breaks and apoptosis in symmetric NPC-NPC divisions, but not in asymmetric neurogenic divisions. This division mode dependence is phenocopied following conditional deletion of HR gene Brca2 . Thus, distinct modes of NPC division have divergent requirements for Ino80 -dependent HR DNA repair. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-020-17551-4 |
| الإتاحة: | https://doi.org/10.1038/s41467-020-17551-4Test https://www.nature.com/articles/s41467-020-17551-4.pdfTest https://www.nature.com/articles/s41467-020-17551-4Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.F5E2B474 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-020-17551-4 |
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