دورية أكاديمية
Production of Kinanthraquinone D with Antimalarial Activity by Heterologous Gene Expression and Biotransformation in Streptomyces lividans TK23
| العنوان: | Production of Kinanthraquinone D with Antimalarial Activity by Heterologous Gene Expression and Biotransformation in Streptomyces lividans TK23 |
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| المؤلفون: | Katsuyuki Sakai, Yushi Futamura, Toshihiko Nogawa, Yuzhu Zhao, Hiroyuki Koshino, Hiroyuki Osada, Shunji Takahashi |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Cell Biology, Pharmacology, Inorganic Chemistry, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, biosynthetic gene cluster, 4 , 3 , two known compounds, streptomyces lividans tk23, heterologous gene expression, 2 , 1 , kinanthraquinone (< b, (< b, lividans tk23, heterologous expression, kiqa <, kiq <, without cytotoxicity, specific regulator, isolated along, experiments indicated, endogenous enzymes, conducted using, chemical structures |
| الوصف: | Two new compounds, kinanthraquinone C ( 1 ) and kinanthraquinone D ( 2 ), were isolated along with two known compounds, kinanthraquinone ( 3 ) and kinanthraquinone B ( 4 ), produced by the heterologous expression of the kiq biosynthetic gene cluster and its pathway-specific regulator, kiqA , in Streptomyces lividans TK23. The chemical structures of compounds 1 and 2 were determined using mass spectrometry and nuclear magnetic resonance analyses. To examine a biosynthetic pathway of compounds 1 and 2 , incubation experiments were conducted using S. lividans TK23 to supply the compounds 3 and 4 . These experiments indicated that compounds 3 and 4 were converted to compounds 2 and 1 , respectively, by the endogenous enzymes of S. lividans TK23. Compounds 2 , 3 , and 4 had antimalarial activities at half-maximal inhibitory concentration values of 0.91, 1.2, and 15 μM, respectively, without cytotoxicity up to 30 μM. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Production_of_Kinanthraquinone_D_with_Antimalarial_Activity_by_Heterologous_Gene_Expression_and_Biotransformation_in_Streptomyces_lividans_TK23/25300976Test |
| DOI: | 10.1021/acs.jnatprod.3c01076.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jnatprod.3c01076.s001Test https://figshare.com/articles/journal_contribution/Production_of_Kinanthraquinone_D_with_Antimalarial_Activity_by_Heterologous_Gene_Expression_and_Biotransformation_in_Streptomyces_lividans_TK23/25300976Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.F59AF493 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jnatprod.3c01076.s001 |
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