دورية أكاديمية
Modulation of protein synthesis in rabbit inner cell mass-derived cells by FGF-2
| العنوان: | Modulation of protein synthesis in rabbit inner cell mass-derived cells by FGF-2 |
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| المؤلفون: | Dvorak, Petr, Campion, Evelyne, Fléchon, Jacques, Hampl, Ales, Renard, Jean |
| المصدر: | ISSN: 0926-5287. |
| بيانات النشر: | HAL CCSD EDP Sciences |
| سنة النشر: | 2001 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | rabbit, inner cell mass, protein synthesis, FGF-2, two-dimensional gel electrophoresis, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.BDD]Life Sciences [q-bio]/Development Biology |
| الوصف: | International audience ; Gastrulation is a critical step in vertebrate development, that depends on synergistic effects of several signalling molecules, including fibroblast growth factor-2 (FGF-2). To follow this phenomenon in vitro we isolated rabbit inner cell masses (ICMs) at embryonic day 4 and we exposed ICM-derived cells to FGF-2. Then, we analysed the quantitative differences in rates of protein synthesis from day 3 to day 5 of culture by two-dimensional (2D) gel electrophoresis. Here we show that both up- and down-regulation of protein synthesis took place in ICM-derived cells upon their exposure to FGF-2. The effect of FGF-2 was most pronounced at day 4 of culture, when the changes were very much in favour of a set of down-regulated proteins. To test the significance of this period of time for FGF-2-mediated regulation of protein synthesis, cells were grown without FGF-2 and then they were pulse-treated with FGF-2 at the end of day 4. When compared to the continuous culture with FGF-2, the FGF-2 pulse resulted in a quite indistinguishable pattern of up- and down-regulated proteins. Thus, the readiness of ICM-derived cells to accept and respond to the FGF-2 signals may be of limited duration. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-00900349; https://hal.science/hal-00900349Test; https://hal.science/hal-00900349/documentTest; https://hal.science/hal-00900349/file/hal-00900349.pdfTest |
| DOI: | 10.1051/rnd:2001123 |
| الإتاحة: | https://doi.org/10.1051/rnd:2001123Test https://hal.science/hal-00900349Test https://hal.science/hal-00900349/documentTest https://hal.science/hal-00900349/file/hal-00900349.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F570CEF2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1051/rnd:2001123 |
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