التفاصيل البيبلوغرافية
| العنوان: |
Phenotype and Antimicrobial Activity ofTh17 Cells Induced by Propionibacterium acnes Strains Associated with Healthy andAcne Skin |
| المؤلفون: |
Agak, George W, Kao, Stephanie, Ouyang, Kelsey, Qin, Min, Moon, David, Butt, Ahsan, Kim, Jenny |
| المصدر: |
Journal of Investigative Dermatology, vol 138, iss 2 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2018 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Biomedical and Clinical Sciences, Immunology, Aetiology, 2.1 Biological and endogenous factors, Infection, Acne Vulgaris, Gram-Positive Bacterial Infections, Humans, Interleukins, Leukocytes, Mononuclear, Limulus Test, Microbial Sensitivity Tests, Microbiota, Propionibacterium acnes, Recombinant Proteins, Skin, Th17 Cells, Clinical Sciences, Oncology and Carcinogenesis, Dermatology & Venereal Diseases |
| جغرافية الموضوع: |
316 - 324 |
| الوصف: |
Studies of the human skin microbiome suggest that Propionibacterium acnes strains may contribute differently to skin health and disease. However, the immune phenotype and functions of T helper type 17 (Th17) cells induced by healthy (PH) versus acne (PA) skin-associated P.acnes strains are currently unknown. We stimulated peripheral blood mononuclear cells from healthy donors and observed that PA strains induce higher IL-17 levels than PH strains. We next generated PH and PA strain-specific Th17 clones and show that P.acnes strains induce Th17 cells of varied phenotype and function that are stable in the presence of IL-2 and IL-23. Although PH- and PA-specific clones expressed similar levels of LL-37 and DEFB4, only PH-specific clones secreted molecules sufficient to kill P.acnes. Furthermore, electron microscopic studies showed that supernatants derived from activated PH and not PA-specific clones exhibited robust bactericidal activity against P.acnes, and complete breaches in the bacterial cell envelope were observed. This antimicrobial activity was independent of IL-26, because both natural IL-26 released by Th17 clones and rhIL-26 lacked antimicrobial potency against P.acnes. Overall, our data suggest that P.acnes strains may differentially modulate the CD4+ T-cell responses, leading to the generation of Th17 cells that may contribute to either homeostasis or acne pathogenesis. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt10s966cj; https://escholarship.org/uc/item/10s966cjTest |
| الإتاحة: |
https://escholarship.org/uc/item/10s966cjTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.F464BBB7 |
| قاعدة البيانات: |
BASE |
