صورة
Bhlhe40 suppresses Chlamydia -specific Tr1 differentiation in a T cell-intrinsic manner.
| العنوان: | Bhlhe40 suppresses Chlamydia -specific Tr1 differentiation in a T cell-intrinsic manner. |
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| المؤلفون: | Miguel A. B. Mercado, Qiang Li, Charles M. Quick, Yejin Kim, Rachel Palmer, Lu Huang, Lin-Xi Li |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Physiology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Biological Sciences not elsewhere classified, regulatory type 1, protein basic helix, female reproductive tract, div >< p, whereas wt cd4, 10r increased cd4, key molecular driver, heightened bacterial burdens, >< sup ><, decreased polyfunctional cd4, >- deficient cd4, exhibited severe defects, chlamydia muridarum <, chlamydia <, polyfunctional responses, key regulator, >, mice deficient, using single |
| الوصف: | (A-C) WT and Bhlhe40 -/- mice were infected intravaginally with 1×10 5 C . muridarum and analyzed at 10 dpi. (A) Representative FACS plots depicting IFNγ- and IL-10-producing CD4 T cells (gated on live CD90.2 + CD4 + CD44 hi cells). (B) Percentages and total cell numbers of IFNγ + , IL-10 + and IFNγ + IL-10 + CD4 T cells within the CD4 + CD44 hi population. (C) Mean fluorescence intensities (MFIs) of IFNγ and IL-10 in CD4 + CD44 hi IFNγ + and CD4 + CD44 hi IL-10 + T cells, respectively. Data are from two independent experiments with 8 mice per group. Each data point represents an individual mouse. (D-H) Mixed WT and Bhlhe40 -/- CD4 T cell adoptive transfer. (D) Experimental workflow. (E) Normalized ratios of Bhlhe40 -/- (CD45.2 + ) and WT (CD45.1 + ) donor CD4 T cells in TCRβ -/- host. Representative FACS plots (F), summary data (G) and MFI (H) of cytokine-producing donor CD4 T cells in TCRβ -/- FRT. Data are from two independent experiments with 6 mice per group. Each pair of data points represents the two donor T cell populations in an individual TCRβ -/- host. (I-K) Chlamydia -specific TP1 CD4 T cell adoptive transfer. (I) Experimental workflow. Representative FACS plots (J) and summary data (K) of cytokine-producing Bhlhe40 -/- TP1 (CD45.2 + CD90.1 - ), WT TP1 (CD45.2 + CD90.1 + ) and B6 host (CD45.2 - CD90.1 - ) CD4 T cells in the host FRT. Data are from two independent experiments with 6 samples per group. Each data point represents a pooled FRT sample from 4 mice. Error bars represent the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant by unpaired t test in (B-C), and paired t test in (E-K). |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/figure/_i_Bhlhe40_i_suppresses_i_Chlamydia_i_-specific_Tr1_differentiation_in_a_T_cell-intrinsic_manner_/25065752Test |
| DOI: | 10.1371/journal.ppat.1011983.g002 |
| الإتاحة: | https://doi.org/10.1371/journal.ppat.1011983.g002Test https://figshare.com/articles/figure/_i_Bhlhe40_i_suppresses_i_Chlamydia_i_-specific_Tr1_differentiation_in_a_T_cell-intrinsic_manner_/25065752Test |
| حقوق: | CC BY 4.0 |
| رقم الانضمام: | edsbas.F455083E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.ppat.1011983.g002 |
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