التفاصيل البيبلوغرافية
| العنوان: |
Single-cell transcriptome analysis of NEUROG3+ cells during pancreatic endocrine differentiation with small molecules |
| المؤلفون: |
Li, Jin, Chen, Junru, Luo, Xiaoyu, Lu, Guangxiu, Lin, Ge |
| المساهمون: |
Natural Science Foundation of Hunan Province |
| المصدر: |
Stem Cell Research & Therapy ; volume 14, issue 1 ; ISSN 1757-6512 |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Cell Biology, Biochemistry, Genetics and Molecular Biology (miscellaneous), Molecular Medicine, Medicine (miscellaneous) |
| الوصف: |
The efficiency of inducing human embryonic stem cells into NEUROG3+ pancreatic endocrine cells is a bottleneck in stem cell therapy for diabetes. To understand the cell properties and fate decisions during differentiation, we analyzed the modified induction method using single-cell transcriptome and found that DAPT combined with four factors (4FS): nicotinamide, dexamethasone, forskolin and Alk5 inhibitor II (DAPT + 4FS) increased the expression of NEUROG3 to approximately 34.3%. The increased NEUROG3+ cells were mainly concentrated in Insulin + Glucagon + (INS + GCG+) and SLAC18A1 + Chromogranin A+(SLAC18A1 + CHGA +) populations, indicating that the increased NEUROG3+ cells promoted the differentiation of pancreatic endocrine cells and enterochromaffin-like cells. Single-cell transcriptome analysis provided valuable clues for further screening of pancreatic endocrine cells and differentiation of pancreatic islet cells. The gene set enrichment analysis (GSEA) suggest that we can try to promote the expression of INS + GCG+ population by up-regulating G protein-coupled receptor (GPCR) and mitogen-activated protein kinase signals and down-regulating Wnt, NIK/NF-KappaB and cytokine-mediated signal pathways. We can also try to regulate GPCR signaling through PLCE1, so as to increase the proportion of NEUROG3+ cells in INS+GCG+ populations. To exclude non-pancreatic endocrine cells, ALCAM high CD9 low could be used as a marker for endocrine populations, and ALCAM high CD9 low CDH1 low could remove the SLC18A1 + CHGA+ population. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1186/s13287-023-03338-z |
| DOI: |
10.1186/s13287-023-03338-z.pdf |
| DOI: |
10.1186/s13287-023-03338-z/fulltext.html |
| الإتاحة: |
https://doi.org/10.1186/s13287-023-03338-zTest |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: |
edsbas.F44D0D8 |
| قاعدة البيانات: |
BASE |
