دورية أكاديمية
TGFβ limits Myc-dependent TCR-induced metabolic reprogramming in CD8+ T cells
| العنوان: | TGFβ limits Myc-dependent TCR-induced metabolic reprogramming in CD8+ T cells |
|---|---|
| المؤلفون: | Hope, HC, Pickersgill, G, Ginefra, P, Vannini, N, Cook, GP, Salmond, RJ |
| بيانات النشر: | Frontiers Media |
| سنة النشر: | 2022 |
| المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| الوصف: | T cell activation is dependent upon the integration of antigenic, co-stimulatory and cytokine-derived signals and the availability and acquisition of nutrients from the environment. Furthermore, T cell activation is accompanied by reprogramming of cellular metabolism to provide the energy and building blocks for proliferation, differentiation and effector function. Transforming growth factor β (TGFβ) has pleiotropic effects on T cell populations, having both an essential role in the maintenance of immune tolerance but also context-dependent pro-inflammatory functions. We set out to define the mechanisms underpinning the suppressive effects of TGFβ on mouse CD8+ T cell activation. RNA-sequencing analysis of TCR-stimulated T cells determined that Myc-regulated genes were highly enriched within gene sets downregulated by TGFβ. Functional analysis demonstrated that TGFβ impeded TCR-induced upregulation of amino acid transporter expression, amino acid uptake and protein synthesis. Furthermore, TCR-induced upregulation of Myc-dependent glycolytic metabolism was substantially inhibited by TGFβ treatment with minimal effects on mitochondrial respiration. Thus, our data suggest that inhibition of Myc-dependent metabolic reprogramming represents a major mechanism underpinning the suppressive effects of TGFβ on CD8+ T cell activation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://eprints.whiterose.ac.uk/190065/1/2022-FrontImmunol.pdfTest; Hope, HC, Pickersgill, G, Ginefra, P et al. (3 more authors) (2022) TGFβ limits Myc-dependent TCR-induced metabolic reprogramming in CD8+ T cells. Frontiers in Immunology, 13. 913184. ISSN 1664-3224 |
| الإتاحة: | https://eprints.whiterose.ac.uk/190065Test/ https://eprints.whiterose.ac.uk/190065/1/2022-FrontImmunol.pdfTest |
| حقوق: | cc_by_4 |
| رقم الانضمام: | edsbas.F42A394 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |