التفاصيل البيبلوغرافية
| العنوان: |
Different levels of the neuronal nitric oxide synthase isoform modulate the rate of osteoclastic differentiation of TIB‐71 and CRL‐2278 RAW 264.7 murine cell clones |
| المؤلفون: |
Nicolin, Vanessa, Ponti, Cristina, Narducci, Paola, Grill, Vittorio, Bortul, Roberta, Zweyer, Marina, Vaccarezza, Mauro, Zauli, Giorgio |
| المصدر: |
The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology ; volume 286A, issue 2, page 945-954 ; ISSN 1552-4884 1552-4892 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2005 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
It has been clearly established that osteoclasts, which play a crucial role in bone resorption, differentiate from hematopoietic cells belonging to the monocyte/macrophage lineage in the presence of macrophage‐colony stimulating factor (M‐CSF) and receptor activator of NF‐κB ligand (RANKL). We have here investigated the M‐CSF‐ and RANKL‐induced osteoclastic differentiation of two distinct clones of the murine monocytic/macrophagic RAW 264.7 cell line, known as TIB‐71 and CRL‐2278, the latter cell clone being defective for the expression of the inducible nitric oxide synthase isoform in response to interferon‐γ or lipopolysaccharide. CRL‐2278 cells demonstrated a more rapid osteoclastic differentiation than TIB‐71 cells, as documented by morphology, tartrate‐resistant acid phosphatase positivity, and bone resorption activity. The enhanced osteoclastic differentiation of CRL‐2278 was accompanied by a higher rate of cells in the S/G2‐M phases of cell cycle as compared to TIB‐71. The analysis of nitric oxide synthase (NOS) isoforms clearly demonstrated that only neuronal NOS was detectable at high levels in CRL‐2278 but not in TIB cells under all tested conditions. Moreover, the broad inhibitor of NOS activity L‐NAME significantly inhibited osteoclastic differentiation of CRL‐2278 cells. Altogether, these results demonstrate that a basal constitutive neuronal NOS activity positively affects the RANKL/M‐CSF‐related osteoclastic differentiation. © 2005 Wiley‐Liss, Inc. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/ar.a.20239 |
| الإتاحة: |
https://doi.org/10.1002/ar.a.20239Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.F413C760 |
| قاعدة البيانات: |
BASE |
