دورية أكاديمية
Characterization of calcium-mobilizing, purinergic P2Y2 receptors in human ovarian cancer cells
| العنوان: | Characterization of calcium-mobilizing, purinergic P2Y2 receptors in human ovarian cancer cells |
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| المؤلفون: | Schultze-Mosgau, Askan, Katzur, Ann C., Arora, Krishan K., Stojilkovic, Stanko S., Diedrich, Klaus, Ortmann, Olaf |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2000 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Ovary and oogenesis |
| الوصف: | In human ovarian EFO-21 and EFO-27 carcinoma cells, extracellular ATP induced a concentration-dependent rise in intracellular calcium concentration ([Ca2+] i ), suggesting the expression of a purinoreceptor. ATP and UTP were equipotent in generating [Ca2+] i signals, followed by ATP-γ-S and ADP, whereas β,γ-ATP, 2 methyl 1 thio-ATP, 3′-o-(4-benzoyl) benzoyl-ATP, AMP, and adenosine were ineffective. This pharmacological profile suggested the presence of the P2Y 2 subtype in both cell types, and this was confirmed by reverse transcription–polymerase chain reaction (RT–PCR) analysis using P2Y 2 primers. ATP-induced [Ca2+] i signals were composed of two phases: an early and extracellular calcium-independent phase, followed by a sustained plateau phase that was dependent on capacitative calcium influx. In addition to the rise in the [Ca2+] i , a time- and concentration-dependent increase in phosphatidylethanol accumulation was observed in ATP-stimulated cells, indicating an increase in phospholipase D activity. RT–PCR analysis identified the expression of a transcript for the phospholipase D-1 subtype of this enzyme. Activation of these receptors by a slowly degradable analogue, ATP-γ-S, attenuated basal and fetal calf serum-induced cell proliferation in a time- and concentration-dependent manner. These results indicate that ATP may act as an extracellular messenger in controlling the ovarian epithelial cell cycle through P2Y 2 receptors. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://molehr.oxfordjournals.org/cgi/content/short/6/5/435Test; http://dx.doi.org/10.1093/molehr/6.5.435Test |
| DOI: | 10.1093/molehr/6.5.435 |
| الإتاحة: | https://doi.org/10.1093/molehr/6.5.435Test http://molehr.oxfordjournals.org/cgi/content/short/6/5/435Test |
| حقوق: | Copyright (C) 2000, Oxford University Press |
| رقم الانضمام: | edsbas.F12054FC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/molehr/6.5.435 |
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