التفاصيل البيبلوغرافية
| العنوان: |
Global Microbiota‐Dependent Histone Acetylation Patterns Are Irreversible and Independent of Short Chain Fatty Acids |
| المؤلفون: |
Saiman, Yedidya, Shen, Ting‐Chin David, Lund, Peder J, Gershuni, Victoria M, Jang, Cholsoon, Patel, Shivali, Jung, Sunhee, Furth, Emma E, Friedman, Elliot S, Chau, Lillian, Garcia, Benjamin A, Wu, Gary D |
| المصدر: |
Hepatology, vol 74, iss 6 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2021 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Digestive Diseases, Genetics, Nutrition, Complementary and Integrative Health, Human Genome, Prevention, Underpinning research, Aetiology, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, Oral and gastrointestinal, Animals, Cell Line, Fatty Acids, Volatile, Gastrointestinal Microbiome, Histone Acetyltransferases, Male, Mice, Inbred C57BL, Medical Biochemistry and Metabolomics, Clinical Sciences, Immunology, Gastroenterology & Hepatology |
| الوقت: |
3427 - 3440 |
| الوصف: |
Background and aimsAlthough germ-free mice are an indispensable tool in studying the gut microbiome and its effects on host physiology, they are phenotypically different than their conventional counterparts. While antibiotic-mediated microbiota depletion in conventional mice leads to physiologic alterations that often mimic the germ-free state, the degree to which the effects of microbial colonization on the host are reversible is unclear. The gut microbiota produce abundant short chain fatty acids (SCFAs), and previous studies have demonstrated a link between microbial-derived SCFAs and global hepatic histone acetylation in germ-free mice.Approach and resultsWe demonstrate that global hepatic histone acetylation states measured by mass spectrometry remained largely unchanged despite loss of luminal and portal vein SCFAs after antibiotic-mediated microbiota depletion. In contrast to stable hepatic histone acetylation states, we see robust hepatic transcriptomic alterations after microbiota depletion. Additionally, neither dietary supplementation with supraphysiologic levels of SCFA nor the induction of hepatocyte proliferation in the absence of microbiota-derived SCFAs led to alterations in global hepatic histone acetylation.ConclusionsThese results suggest that microbiota-dependent landscaping of the hepatic epigenome through global histone acetylation is static in nature, while the hepatic transcriptome is responsive to alterations in the gut microbiota. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt6cz139vj; https://escholarship.org/uc/item/6cz139vjTest |
| الإتاحة: |
https://escholarship.org/uc/item/6cz139vjTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.F0D40A96 |
| قاعدة البيانات: |
BASE |
