التفاصيل البيبلوغرافية
| العنوان: |
Simultaneous enrichment of plasma soluble and extracellular vesicular glycoproteins using prolonged ultracentrifugation-electrostatic repulsion-hydrophilic interaction chromatography (PUC-ERLIC) approach |
| المؤلفون: |
Cheow, Esther Sok Hwee, Sim, Kae Hwan, De Kleijn, Dominique, Lee, Chuen Neng, Sorokin, Vitaly, Sze, Siu Kwan |
| المساهمون: |
Verpleegafd Vaatchirurgie D4 Oost, UMC Utrecht, Infection & Immunity, Circulatory Health, Onderzoek Vrouw Hart & Vaatziekten |
| سنة النشر: |
2015 |
| مصطلحات موضوعية: |
Biochemistry, Molecular Biology, Analytical Chemistry |
| الوصف: |
Plasma glycoproteins and extracellular vesicles represent excellent sources of disease biomarkers, but laboratory detection of these circulating structures are limited by their relatively low abundance in complex biological fluids. Although intensive research has led to the development of effective methods for the enrichment and isolation of either plasma glycoproteins or extracellular vesicles from clinical materials, at present it is not possible to enrich both structures simultaneously from individual patient sample, a method that affords the identification of biomarker combinations from both entities for the prediction of clinical outcomes will be clinically useful. We have therefore developed an enrichment method for use in mass spectrometry-based proteomic profiling that couples prolonged ultracentrifugation with electrostatic repulsion- hydrophilic interaction chromatography, to facilitate the recovery of both glycoproteins and extracellular vesicles from nondepleted human plasma. Following prolonged ultracentrifugation, plasma glycoproteins and extracellular vesicles were concentrated as a yellow suspension, and simultaneous analyses of low abundant secretory and vesicular glycoproteins was achieved in a single LC-MS/MS run. Using this systematic prolonged ultracentrifugation-electrostatic repulsion-hydrophilic interaction chromatography approach, we identified a total of 127 plasma glycoproteins at a high level of confidence (FDR ≤ 1%), including 48 glycoproteins with concentrations ranging from pg to ng/ml. The novel enrichment method we report should facilitate future human plasmabased proteome and glycoproteome that will identify novel biomarkers, or combinations of secreted and vesicle- derived biomarkers, that can be used to predict clinical outcomes in human patients. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1535-9476 |
| العلاقة: |
https://dspace.library.uu.nl/handle/1874/350314Test |
| الإتاحة: |
https://dspace.library.uu.nl/handle/1874/350314Test |
| حقوق: |
info:eu-repo/semantics/ClosedAccess |
| رقم الانضمام: |
edsbas.F09C98AB |
| قاعدة البيانات: |
BASE |
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