التفاصيل البيبلوغرافية
| العنوان: |
RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis |
| المؤلفون: |
Aleksandra Głowacka, Ewa Kilańczyk, Małgorzata Maksymowicz, Małgorzata Zawadzka, Wiesława Leśniak, Anna Filipek |
| المصدر: |
International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 5969 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
MO3.13 cells, differentiation, phorbol 12-myristate 13-acetate, oligodendrocytes, RNA polymerase I (Pol I) inhibitor, p53 activity, myogenesis, K-Ras signaling |
| جغرافية الموضوع: |
agris |
| الوصف: |
In this work, we examined the differentiation of oligodendrocytic MO3.13 cells and changes in their gene expression after treatment with phorbol 12-myristate 13-acetate, PMA, or with RNA polymerase I (Pol I) inhibitor, CX-5461. We found that MO3.13 cells changed their morphology when treated with both agents. Interestingly, CX-5461, but not PMA, induced noticeable changes in the integrity of the nucleoli. Then, we analyzed the p53 transcriptional activity in MO3.13 cells and found that it was increased in both cell populations, but particularly in cells treated with PMA. Interestingly, this high p53 transcriptional activity in PMA-treated cells coincided with a lower level of an unmodified (non-phosphorylated) form of this protein. Since morphological changes in MO3.13 cells after PMA and CX-5461 treatment were evident, suggesting that cells were induced to differentiate, we performed RNA-seq analysis of PMA-treated cells, to reveal the direction of alterations in gene expression. The analysis showed that the largest group of upregulated genes consisted of those involved in myogenesis and K-RAS signaling, rather than those associated with oligodendrocyte lineage progression. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Molecular Genetics and Genomics; https://dx.doi.org/10.3390/ijms23115969Test |
| DOI: |
10.3390/ijms23115969 |
| الإتاحة: |
https://doi.org/10.3390/ijms23115969Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.F02A48A6 |
| قاعدة البيانات: |
BASE |
