تقرير
Isolation of mouse mammary carcinoma-derived macrophages and cancer cells for co-culture assays
| العنوان: | Isolation of mouse mammary carcinoma-derived macrophages and cancer cells for co-culture assays |
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| المؤلفون: | Sun, Lijuan, Han, Xiao, Egeblad, Mikala |
| سنة النشر: | 2022 |
| المجموعة: | Cold Spring Harbor Laboratory: CSHL Institutional Repository |
| مصطلحات موضوعية: | cancer, Investigative techniques and equipment, animal, assays, breast cancer, cell culture, cell types, cell types and functions, macrophages, mammal, mouse, organs, tissues, organelles, rodent, cancer types |
| الوصف: | We recently established an in vitro co-culture system in which monophosphoryl lipid A + interferon-γ (MPLA+IFNγ)-treated tumor-associated macrophages (TAMs) killed cancer cells. Here, we describe a step-by-step protocol for isolating TAMs and cancer cells from mouse primary mammary carcinomas, the setup of the co-culture system, and the image acquisition approach. The technical difficulties in the co-culture assay involve isolating pure TAMs and cancer cells from the same tumor and staining them with different dyes to track the macrophages' tumoricidal activity. For complete details on the use and execution of this protocol, please refer to Sun et al.(2021). |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://repository.cshl.edu/id/eprint/40760/1/2022-Egeblad-Isolation-of-mouse-mammary-carcinoma-derived-macrophages-and-cancer-cells-for-co-culture-assays.pdfTest; Sun, Lijuan, Han, Xiao, Egeblad, Mikala (December 2022) Isolation of mouse mammary carcinoma-derived macrophages and cancer cells for co-culture assays. STAR Protocols, 3 (4). p. 101833. ISSN 2666-1667 (In Press) |
| DOI: | 10.1016/j.xpro.2022.101833 |
| الإتاحة: | https://doi.org/10.1016/j.xpro.2022.101833Test https://repository.cshl.edu/id/eprint/40760Test/ https://repository.cshl.edu/id/eprint/40760/1/2022-Egeblad-Isolation-of-mouse-mammary-carcinoma-derived-macrophages-and-cancer-cells-for-co-culture-assays.pdfTest https://pubmed.ncbi.nlm.nih.gov/36386879Test/ |
| حقوق: | cc_by_nc_nd |
| رقم الانضمام: | edsbas.EFFDBB8C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.xpro.2022.101833 |
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