دورية أكاديمية
Alginate microbeads as potential support for cultivation of bone marrow stromal cells
| العنوان: | Alginate microbeads as potential support for cultivation of bone marrow stromal cells |
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| المؤلفون: | Bugarski, Diana, Obradović, B., Petakov, M, Jovcić, G, Stojanović, N, Bugarski, Branko |
| المصدر: | Materials Science Forum |
| بيانات النشر: | Current Research in Advanced Materials and Processes |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | alginate, bone marrow stromal cells, tissue cultivation |
| الوصف: | Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| ردمك: | 978-0-00-230985-1 0-00-230985-8 |
| تدمد: | 0255-5476 |
| العلاقة: | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/712Test; 2-s2.0-35248894817; 000230985800086 |
| DOI: | 10.4028/0-87849-971-7.525 |
| الإتاحة: | https://doi.org/10.4028/0-87849-971-7.525Test http://TechnoRep.tmf.bg.ac.rs/handle/123456789/712Test |
| حقوق: | restrictedAccess ; ARR |
| رقم الانضمام: | edsbas.EEDAE8B6 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780002309851 0002309858 |
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| تدمد: | 02555476 |
| DOI: | 10.4028/0-87849-971-7.525 |