دورية أكاديمية
IFN-c Upregulates Survivin and Ifi202 Expression to Induce Survival and Proliferation of Tumor-Specific T Cells
العنوان: | IFN-c Upregulates Survivin and Ifi202 Expression to Induce Survival and Proliferation of Tumor-Specific T Cells |
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المؤلفون: | Zimmerman, Mary, Yang, Dafeng, Hu, Xiaolin, Liu, Feiyan, Singh, Nagendra, Browning, Darren, Ganapathy, Vadivel, Chandler, Phillip, Choubey, Divaker, Abrams, Scott I., Liu, Kebin |
المساهمون: | Department of Biochemistry and Molecular Biology, Immunotherapy Center |
سنة النشر: | 2010 |
المجموعة: | Augusta University: Scholarly Commons |
مصطلحات موضوعية: | Research Article, Immunology, Immunology/Genetics of the Immune System, Immunology/Immune Response, Immunology/Immunomodulation, Immunology/Leukocyte Activation, Immunology/Leukocyte Development, Animals, Antibodies, Monoclonal, Antigens, CD3, Apoptosis, Blotting, Western, Cell Line, Tumor, Cell Proliferation, Cell Survival, Female, Gene Expression Profiling, Immunotherapy, Adoptive, Inhibitor of Apoptosis Proteins, Interferon-gamma, Intracellular Signaling Peptides and Proteins, Mice, Inbred BALB C, Neoplasms, Experimental |
الوصف: | Background: A common procedure in human cytotoxic T lymphocyte (CTL) adoptive transfer immunotherapy is to expand tumor-specific CTLs ex vivo using CD3 mAb prior to transfer. One of the major obstacles of CTL adoptive immunotherapy is a lack of CTL persistence in the tumor-bearing host after transfer. The aim of this study is to elucidate the molecular mechanisms underlying the effects of stimulation conditions on proliferation and survival of tumor-specific CTLs. ; Methodology/Principal Findings: Tumor-specific CTLs were stimulated with either CD3 mAb or cognate Ag and analyzed for their proliferation and survival ex vivo and persistence in tumor-bearing mice. Although both Ag and CD3 mAb effectively induced the cytotoxic effecter molecules of the CTLs, we observed that Ag stimulation is essential for sustained CTL proliferation and survival. Further analysis revealed that Ag stimulation leads to greater proliferation rates and less apoptosis than CD3 mAb stimulation. Re-stimulation of the CD3 mAb-stimulated CTLs with Ag resulted in restored CTL proliferative potential, suggesting that CD3 mAb-induced loss of proliferative potential is reversible. Using DNA microarray technology, we identified that survivin and ifi202, two genes with known functions in T cell apoptosis and proliferation, are differentially induced between Ag- and CD3 mAb-stimulated CTLs. Analysis of the IFN-γ signaling pathway activation revealed that Ag stimulation resulted in rapid phosphorylation of STAT1 (pSTAT1), whereas CD3 mAb stimulation failed to activate STAT1. Chromatin immunoprecipitation revealed that pSTAT1 is associated with the promoters of both survivin and ifi202 in T cells and electrophoresis mobility shift assay indicated that pSTAT1 directly binds to the gamma activation sequence element in the survivin and ifi202 promoters. Finally, silencing ifi202 expression significantly decreased T cell proliferation. ; Conclusions/Significance: Our findings delineate a new role of the IFN-γ signaling pathway in regulating T cell ... |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | unknown |
تدمد: | 1932-6203 21124930 |
العلاقة: | PLoS One. 2010 Nov 22; 5(11):e14076; http://hdl.handle.net/10675.2/610Test; PMC2989915 |
DOI: | 10.1371/journal.pone.0014076 |
الإتاحة: | https://doi.org/10.1371/journal.pone.0014076Test http://hdl.handle.net/10675.2/610Test |
حقوق: | Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
رقم الانضمام: | edsbas.ECCF9946 |
قاعدة البيانات: | BASE |
تدمد: | 19326203 21124930 |
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DOI: | 10.1371/journal.pone.0014076 |