تقرير
Competition between transcription and loop extrusion modulates promoter and enhancer dynamics
| العنوان: | Competition between transcription and loop extrusion modulates promoter and enhancer dynamics |
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| المؤلفون: | Platania, Angeliki, Erb, Cathie, Barbieri, Mariano, Molcrette, Bastien, Grandgirard, Erwan, de Kort, Marit Ac, Meaburn, Karen, Taylor, Tiegh, Shchuka, Virlana, M, Kocanova, Silvia, Monteiro Oliveira, Guilherme, Mitchell, Jennifer, A, Soutoglou, Evi, Lenstra, Tineke, L, Molina, Nacho, Papantonis, Argyris, Bystricky, Kerstin, Sexton, Tom |
| المساهمون: | Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de biologie Moléculaire, Cellulaire et du Développement (MCD), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institute of Pathology - University Medical Centre Göttingen, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), ANR-10-IDEX-0002,UNISTRA,Par-delà les frontières, l'Université de Strasbourg(2010), ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), ANR-10-LABX-0030,INRT,Integrative Biology : Nuclear dynamics- Regenerative medicine - Translational medicine(2010) |
| المصدر: | https://hal.science/hal-04312310Test ; 2023. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2023 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | The spatiotemporal configuration of genes with distal regulatory elements, and the impact of chromatin mobility on transcription, remain unclear. Loop extrusion is an attractive model for bringing genetic elements together, but how this functionally interacts with transcription is also largely unknown. We combine live tracking of genomic loci and nascent transcripts with molecular dynamics simulations to assess the 4D arrangement of the Sox2 gene and its enhancer, in response to a battery of perturbations. We find that alterations in chromatin mobility, not promoter-enhancer distance, is more informative about transcriptional status. Active elements display more constrained mobility, consistent with confinement within specialized nuclear sites, and alterations in enhancer mobility distinguish poised from transcribing alleles. Strikingly, we find that whereas loop extrusion and transcription factor-mediated clustering contribute to promoter-enhancer proximity, they have antagonistic effects on chromatin dynamics. This provides an experimental framework for the underappreciated role of chromatin dynamics in genome regulation. |
| نوع الوثيقة: | report |
| اللغة: | English |
| العلاقة: | hal-04312310; https://hal.science/hal-04312310Test; https://hal.science/hal-04312310/documentTest; https://hal.science/hal-04312310/file/2023.04.25.538222v1.full.pdfTest; BIORXIV: 2023.04.25.538222 |
| DOI: | 10.1101/2023.04.25.538222 |
| الإتاحة: | https://doi.org/10.1101/2023.04.25.538222Test https://hal.science/hal-04312310Test https://hal.science/hal-04312310/documentTest https://hal.science/hal-04312310/file/2023.04.25.538222v1.full.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.EAECE234 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2023.04.25.538222 |
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