دورية أكاديمية
Hsa-miR-139-5p is a prognostic thyroid cancer marker involved in HNRNPF-mediated alternative splicing.
| العنوان: | Hsa-miR-139-5p is a prognostic thyroid cancer marker involved in HNRNPF-mediated alternative splicing. |
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| المؤلفون: | Montero-Conde, Cristina, Graña-Castro, Osvaldo, Martín-Serrano, Guillermo, Martínez-Montes, Ángel M, Zarzuela, Eduardo, Muñoz, Javier, Torres-Perez, Rafael, Pita, Guillermo, Cordero-Barreal, Alfonso, Leandro-García, Luis J, Letón, Rocío, López de Silanes, Isabel, Guadalix, Sonsoles, Pérez-Barrios, Andrés, Hawkins, Federico, Guerrero-Álvarez, Almudena, Álvarez-Escolá, Cristina, Regojo-Zapata, Rita M, Calsina, Bruna, Remacha, Laura, Roldán-Romero, Juan M, Santos, María, Lanillos, Javier, Jordá, Mireia, Riesco-Eizaguirre, Garcilaso, Zafon, Carles, González-Neira, Anna, Blasco, MA, Al-Shahrour, Fatima, Rodríguez-Antona, Cristina, Cascon Soriano, Alberto, Robledo Batanero, Mercedes |
| المساهمون: | Asociación Española Contra el Cáncer, Instituto de Salud Carlos III, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) |
| بيانات النشر: | Wiley |
| سنة النشر: | 2020 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | Gene Expression Regulation, Neoplastic, Adult, Aged, 80 and over, Alternative Splicing, Biomarkers, Tumor, Cell Line, Cell Proliferation, Disease-Free Survival, Female, Follow-Up Studies, Gene Expression Profiling, Heterogeneous-Nuclear Ribonucleoprotein Group F-H, Humans, Kaplan-Meier Estimate, Male, MicroRNAs, Middle Aged, Prognosis, Signal Transduction, Survival Rate, Thyroid Gland, Thyroid Neoplasms |
| الوصف: | It is critical to identify biomarkers and functional networks associated with aggressive thyroid cancer to anticipate disease progression and facilitate personalized patient management. We performed miRNome sequencing of 46 thyroid tumors enriched with advanced disease patients with a median follow-up of 96 months. MiRNome profiles correlated with tumor-specific histopathological and molecular features, such as stromal cell infiltration and tumor driver mutation. Differential expression analysis revealed a consistent hsa-miR-139-5p downexpression in primary carcinomas from patients with recurrent/metastatic disease compared to disease-free patients, sustained in paired local metastases and validated in publicly available thyroid cancer series. Exogenous expression of hsa-miR-139-5p significantly reduced migration and proliferation of anaplastic thyroid cancer cells. Proteomic analysis indicated RICTOR, SMAD2/3 and HNRNPF as putative hsa-miR-139-5p targets in our cell system. Abundance of HNRNPF mRNA, encoding an alternative splicing factor involved in cryptic exon inclusion/exclusion, inversely correlated with hsa-miR-139-5p expression in human tumors. RNA sequencing analysis revealed 174 splicing events differentially regulated upon HNRNPF repression in our cell system, affecting genes involved in RTK/RAS/MAPK and PI3K/AKT/MTOR signaling cascades among others. These results point at the hsa-miR-139-5p/HNRNPF axis as a novel regulatory mechanism associated with the modulation of major thyroid cancer signaling pathways and tumor virulence. ; CAM; Grant numbers: S2017/BMD-3724, TIRONET2-CM; Grant sponsor: Fundacion Cientifica Asociacion Espanola Contra el Cancer; Grant number: AIO15152858; Grant sponsor: Instituto de Salud Carlos III (ISCIII), Accion Estrategica en Salud, cofinanciado a traves del Fondo Europeo de Desarrollo Regional (FEDER); Grant numbers: PI14/00240, PI17/01796 ; Sí |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1097-0215 |
| العلاقة: | https://doi.org/10.1002/ijc.32622Test.; info:eu-repo/grantAgreement/ES/PI14/00240; info:eu-repo/grantAgreement/ES/PI17/01796; Int J Cancer . 2020 ;146(2):521-530.; http://hdl.handle.net/20.500.12105/18534Test; International journal of cancer |
| DOI: | 10.1002/ijc.32622 |
| الإتاحة: | https://doi.org/20.500.12105/18534Test https://doi.org/10.1002/ijc.32622Test https://hdl.handle.net/20.500.12105/18534Test |
| حقوق: | http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; open access |
| رقم الانضمام: | edsbas.EA18B237 |
| قاعدة البيانات: | BASE |
| تدمد: | 10970215 |
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| DOI: | 10.1002/ijc.32622 |