التفاصيل البيبلوغرافية
| العنوان: |
Overview of in vitro approaches to investigate chemical contaminants intestinal transport |
| المؤلفون: |
Alves de Sousa, B., Faria, M., Miralles, B., Alvito, Paula |
| سنة النشر: |
2024 |
| المجموعة: |
National Health Institute, Portugal: Repositório Científico |
| مصطلحات موضوعية: |
Toxicologia, Segurança Alimentar, Contaminantes Alimentares, Saúde Humana |
| الوصف: |
The occurrence of chemical contaminants in food products is a serious threat to public health and can lead from mild gastroenteritis to fatal liver, kidney and neurological syndromes. The main route of exposure to chemical contaminants is ingestion, with the intestine being the first organ to come into contact with these compounds. In vitro intestinal cell assays may constitute a useful tool to predict the impact of chemical contaminants on the intestinal barrier. Differentiated Caco-2 cells cultivated in porous Transwell inserts, as an in vitro model to evaluate the intestinal transport of food chemical contaminants, is recurrent. These toxicants can be transported via transcellular (transport that occurs through the epithelial cell) or paracellular (transport that occurs in between cells) pathway. This study aims to make an overview of in vitro approaches to investigate chemical contaminants intestinal transport and associated pathways. A bibliographical search was carried out in two databases of scientific publications, “PUBMED” and “WEB OF SCIENCE”, using the keywords “food”, “chemical contaminants”, “cell transport”, in scientific publications from 1999 to 2021. Forty publications were used for data extraction. In sum, Caco-2 cells were the most frequently used model for the evaluation of the food contaminants intestinal transport. In a few works, this cell line was combined with the human HT-29 cells. In other studies, specifically evaluating the implication of membrane transporters, Madin-Darby canine kidney (MDCK) cells were used. HTC-8 intestinal organoids were also reported and gastric absorption of food toxicants was described in a single work using NCI-N87 cells. The use intestinal mucosa explants mounted in Ussing chambers, in studies of food contaminants intestinal transport in farmed animals, was also reported, although in much less extension. The transcellular route was the most reported pathway for chemical contaminants, with paracellular being less referred (mycotoxins and chloropropanols). ... |
| نوع الوثيقة: |
conference object |
| اللغة: |
English |
| العلاقة: |
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-TOX%2F28762%2F2017/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0094%2F2020/PT; http://hdl.handle.net/10400.18/8821Test |
| الإتاحة: |
http://hdl.handle.net/10400.18/8821Test |
| حقوق: |
closedAccess |
| رقم الانضمام: |
edsbas.E9CDD4FD |
| قاعدة البيانات: |
BASE |
