دورية أكاديمية
Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
| العنوان: | Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes |
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| المؤلفون: | Carvajal, Silvia, Perramón, Meritxell, Casals, Gregori, Oró, Denise, Ribera, Jordi, Morales-Ruiz, Manuel, Casals, Eudald, Casado, Pedro, Melgar-Lesmes, Pedro, Fernández-Varo, Guillermo, Cutillas, Pedro, Puntes, Víctor F., Jiménez, Wladimiro |
| المساهمون: | Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, European Commission, Fundació La Marató de TV3, Wuyi University, Natural Science Foundation of Guangdong Province, Instituto de Salud Carlos III, Perramón, Meritxell, Casals, Gregori, Ribera, Jordi, Casals, Eudald, Jiménez, Wladimiro |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Cerium oxide nanoparticles, Phosphoproteomics, Oxidative stress, Human hepatic cells |
| الوصف: | This article belongs to the Special Issue Innovative Molecular Target and Therapeutic Approaches in Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis (NAFLD/NASH). ; Cerium oxide nanoparticles (CeO2NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO2NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO2NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO2NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO2NPs did not modify HepG2 cell viability in basal conditions but reduced H2O2- and lipopolysaccharide (LPS)-induced cell death and prevented H2O2-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO2NPs reverted the H2O2-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H2O2 effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO2NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways. ; This research was funded by grants to W. Jiménez from Ministerio de Economia y Competitividad [grants, SAF2015-64126-R, RTI2018-094734-B-C21], to M. Morales-Ruiz [grant SAF2016-75358-R] and to G. Casals [grant PI-15/00777]; cofinanced by FEDER, European Union, a way of making Europe, Agència de Gestió d’Ajuts Universitaris i de Recerca [grant SGR 2014/219]; and Fundació La Marató de TV3 [grant ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-2-01-809473-8 2-01-809473-4 |
| تدمد: | 1661-6596 1422-0067 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2015-64126-R; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094734-B-C21; RTI2018-094734-B-C21/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75358-R; Publisher's version; https://doi.org/10.3390/ijms20235959Test; Sí; International Journal of Molecular Sciences 20(23): 5959 (2019); http://hdl.handle.net/10261/209051Test; http://dx.doi.org/10.13039/501100002809Test; http://dx.doi.org/10.13039/501100003329Test; http://dx.doi.org/10.13039/501100011033Test; http://dx.doi.org/10.13039/501100000780Test; http://dx.doi.org/10.13039/100008666Test; http://dx.doi.org/10.13039/501100004587Test |
| DOI: | 10.3390/ijms20235959 |
| DOI: | 10.13039/501100002809 |
| DOI: | 10.13039/501100003329 |
| DOI: | 10.13039/501100011033 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/100008666 |
| DOI: | 10.13039/501100004587 |
| الإتاحة: | https://doi.org/10.3390/ijms20235959Test https://doi.org/10.13039/501100002809Test https://doi.org/10.13039/501100003329Test https://doi.org/10.13039/501100011033Test https://doi.org/10.13039/501100000780Test https://doi.org/10.13039/100008666Test https://doi.org/10.13039/501100004587Test http://hdl.handle.net/10261/209051Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.E9C8968C |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9782018094738 2018094734 |
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| تدمد: | 16616596 14220067 |
| DOI: | 10.3390/ijms20235959 |