دورية أكاديمية
Muscarinic Acetylcholine Receptors Activate TRPC6 Channels in PC12D Cells via Ca2+ Store-Independent Mechanisms
| العنوان: | Muscarinic Acetylcholine Receptors Activate TRPC6 Channels in PC12D Cells via Ca2+ Store-Independent Mechanisms |
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| المؤلفون: | Zhang, Lei, Guo, Feifan, Kim, Ju Young, Saffen, David |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2006 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Regular Papers |
| الوصف: | In this paper we report that stimulation of mAChRs in PC12D cells activates Ca2+ channels that are regulated independently of intracellular Ca2+ stores. In nominally Ca2+-free medium, exposure of PC12D cells to carbachol stimulates a robust influx of Ba2+, a Ca2+ substitute. This influx is blocked by atropine, but not by inhibitors of the nicotinic acetylcholine receptor or L-, N-, or T-type voltage-regulated Ca2+ channels. By contrast, depletion of intracellular Ca2+ stores with thapsigargin only weakly stimulates Ba2+ influx. Unlike store-operated Ca2+ channels (SOCCs), which close only after intracellular Ca2+ stores refill, channels mediating carbachol-stimulated Ba2+ influx rapidly close following the inactivation of mAChRs with atropine. Ba2+ influx is inhibited by extracellular Ca2+, by the Ca2+ channel blocker SKF-96365, and by activation of protein kinase C (PKC). Exogenous expression of antisense RNA encoding the rat c anonical- t ransient r eceptor p otential Ca2+ channel subtype 6 (TRPC6) or the N-terminal domain of TRPC6 blocks carbachol-stimulated Ba2+ influx in PC12D cells. Expression of TRPC6 antisense RNA or the TRPC6 N-terminal domain also blocks Ba2+ influx stimulated by 1-oleoyl-2-acetyl- sn -glycerol (OAG), a diacylglycerol analog previously shown to activate exogenously expressed TRPC6 channels. These data show that mAChRs in PC12D cells activate endogenous Ca2+ channels that are regulated independently of Ca2+ stores and require the expression of TRPC6. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://jb.oxfordjournals.org/cgi/content/short/139/3/459Test; http://dx.doi.org/10.1093/jb/mvj065Test |
| DOI: | 10.1093/jb/mvj065 |
| الإتاحة: | https://doi.org/10.1093/jb/mvj065Test http://jb.oxfordjournals.org/cgi/content/short/139/3/459Test |
| حقوق: | Copyright (C) 2006, Japanese Biochemical Society |
| رقم الانضمام: | edsbas.E852AAE3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jb/mvj065 |
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