دورية أكاديمية
Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 γ–σ1 and AP-3 δ–σ3 hemicomplexes
| العنوان: | Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 γ–σ1 and AP-3 δ–σ3 hemicomplexes |
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| المؤلفون: | Janvier, Katy, Kato, Yukio, Boehm, Markus, Rose, Jeremy R., Martina, José A., Kim, Bong-Yoon, Venkatesan, Sundararajan, Bonifacino, Juan S. |
| المصدر: | The Journal of Cell Biology ; volume 163, issue 6, page 1281-1290 ; ISSN 1540-8140 0021-9525 |
| بيانات النشر: | Rockefeller University Press |
| سنة النشر: | 2003 |
| الوصف: | The sorting of transmembrane proteins to endosomes and lysosomes is mediated by signals present in the cytosolic tails of the proteins. A subset of these signals conform to the [DE]XXXL[LI] consensus motif and mediate sorting via interactions with heterotetrameric adaptor protein (AP) complexes. However, the identity of the AP subunits that recognize these signals remains controversial. We have used a yeast three-hybrid assay to demonstrate that [DE]XXXL[LI]-type signals from the human immunodeficiency virus negative factor protein and the lysosomal integral membrane protein II interact with combinations of the γ and σ1 subunits of AP-1 and the δ and σ3 subunits of AP-3, but not the analogous combinations of AP-2 and AP-4 subunits. The sequence requirements for these interactions are similar to those for binding to the whole AP complexes in vitro and for function of the signals in vivo. These observations reveal a novel mode of recognition of sorting signals involving the γ/δ and σ subunits of AP-1 and AP-3. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1083/jcb.200307157 |
| الإتاحة: | https://doi.org/10.1083/jcb.200307157Test https://rupress.org/jcb/article-pdf/163/6/1281/1527644/jcb16361281.pdfTest |
| رقم الانضمام: | edsbas.E7D29743 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1083/jcb.200307157 |
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