دورية أكاديمية
Genetic and pharmacological inhibition of XBP1 protects against APAP hepatotoxicity through the activation of autophagy
| العنوان: | Genetic and pharmacological inhibition of XBP1 protects against APAP hepatotoxicity through the activation of autophagy |
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| المؤلفون: | Hui Ye1,2,3,23, Chaobo Chen1,2,4,5,23, Hanghang Wu1,23, Kang Zheng1,2,3, Beatriz Martín-Adrados1,2, Esther Caparros6,7,8, Rubén Francés6,7,8, Leonard J. Nelson9, Manuel Gómez del Moral10, Iris Asensio8,11,12, Javier Vaquero8,11,12, Rafael Bañares8,11,12, Matías A. Ávila8,13,14, Raúl J. Andrade8,15, M. Isabel Lucena8,15, Maria Luz Martínez-Chantar 8,16, Helen L. Reeves 17,18, Steven Masson 17,18, Richard S. Blumberg 19, Jordi Gracia-Sancho 8,20,21, Yulia A. Nevzorova1,8,12,22, Eduardo Martínez-Naves1,2, Francisco Javier Cubero 1,8,12✉ |
| سنة النشر: | 2022 |
| المجموعة: | Zenodo |
| الوصف: | Acetaminophen (APAP) hepatotoxicity induces endoplasmic reticulum (ER) stress which triggers the unfolded protein response (UPR) in hepatocytes. However, the mechanisms underlying ER stress remain poorly understood, thus reducing the options for exploring new pharmacological therapies for patients with hyperacute liver injury. Eight-to-twelve-week-old C57BL/6J Xbp1-floxed (Xbp1f/f) and hepatocyte-specific knockout Xbp1 mice (Xbp1Δhepa) were challenged with either high dose APAP [500 mg/kg] and sacrificed at early (1–2 h) and late (24 h) stages of hepatotoxicity. Histopathological examination of livers, immunofluorescence and immunohistochemistry, Western blot, real time (RT)-qPCR studies and transmission electron microscopy (TEM) were performed. Pharmacological inhibition of XBP1 using pre-treatment with STF-083010 [STF, 75 mg/kg] and autophagy induction with Rapamycin [RAPA, 8 mg/kg] or blockade with Chloroquine [CQ, 60 mg/kg] was also undertaken in vivo. Cytoplasmic expression of XBP1 coincided with severity of human and murine hyperacute liver injury. Transcriptional and translational activation of the UPR and sustained activation of JNK1/2 were major events in APAP hepatotoxicity, both in a human hepatocytic cell line and in a preclinical model. Xbp1Δhepa livers showed decreased UPR and JNK1/2 activation but enhanced autophagy in response to high dose APAP. Additionally, blockade of XBP1 splicing by STF, mitigated APAP-induced liver injury and without non-specific off-target effects (e.g., CYP2E1 activity). Furthermore, enhanced autophagy might be responsible for modulating CYP2E1 activity in Xbp1Δhepa animals. Genetic and pharmacological inhibition of Xbp1 specifically in hepatocytes ameliorated APAP-induced liver injury by enhancing autophagy and decreasing CYP2E1 expression. These findings provide the basis for the therapeutic restoration of ER stress and/or induction of autophagy in patients with hyperacute liver injury. Cell Death and Disease (2022) 13:143 ; https://doi.org/10.1038/s41419-022-04580-8Test |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://zenodo.org/record/7125373Test; https://doi.org/10.5281/zenodo.7125373Test; oai:zenodo.org:7125373 |
| DOI: | 10.5281/zenodo.7125373 |
| الإتاحة: | https://doi.org/10.5281/zenodo.7125373Test https://doi.org/10.5281/zenodo.7125372Test https://zenodo.org/record/7125373Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0/legalcodeTest |
| رقم الانضمام: | edsbas.E75F3748 |
| قاعدة البيانات: | BASE |
| DOI: | 10.5281/zenodo.7125373 |
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