دورية أكاديمية
A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system.
| العنوان: | A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system. |
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| المؤلفون: | Boneca, Ivo G, Dussurget, Olivier, Cabanes, Didier, Nahori, Marie-Anne, Sousa, Sandra, Lecuit, Marc, Psylinakis, Emmanuel, Bouriotis, Vassilis, Hugot, Jean-Pierre, Giovannini, Marco, Coyle, Anthony, Bertin, John, Namane, Abdelkader, Rousselle, Jean-Claude, Cayet, Nadège, Prévost, Marie-Christine, Balloy, Viviane, Chignard, Michel, Philpott, Dana J, Cossart, Pascale, Girardin, Stephen E |
| المساهمون: | Pathogénie Bactérienne des Muqueuses, Institut Pasteur Paris, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur Paris -Institut National de la Santé et de la Recherche Médicale (INSERM), Protéomique (Plate-Forme), Enzyme Biotechnology group, University of Crete Heraklion (UOC), Institute of Molecular Biology and Biotechnology, Pharmacogénétique et abords thérapeutiques des maladies héréditaires, Institut National de la Santé et de la Recherche Médicale (INSERM), Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Millennium Pharmaceuticals, Microscopie électronique (Plate-forme), Défense innée et inflammation, Institut Pasteur Paris -Institut National de la Santé et de la Recherche Médicale (INSERM), Immunité Innée et Signalisation, Pathogénie Microbienne Moléculaire |
| المصدر: | ISSN: 0027-8424. |
| بيانات النشر: | HAL CCSD National Academy of Sciences |
| سنة النشر: | 2007 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Acetylation, Amidohydrolases, Animals, Gram-Positive Bacterial Infections, Humans, Immunity, Natural, Interleukin-6, Listeria, Macrophages, Mice, Muramidase, Peptidoglycan, Spectrometry, Mass, Cell Line, Cell Survival, Immune System, Mutation, Matrix-Assi, MESH: Acetylation, MESH: Amidohydrolases, MESH: Listeria, MESH: Macrophages, MESH: Mice, MESH: Muramidase, MESH: Mutation, MESH: Peptidoglycan, MESH: Spectrometry, MESH: Animals |
| الوصف: | Listeria monocytogenes is a human intracellular pathogen that is able to survive in the gastrointestinal environment and replicate in macrophages, thus bypassing the early innate immune defenses. Peptidoglycan (PG) is an essential component of the bacterial cell wall readily exposed to the host and, thus, an important target for the innate immune system. Characterization of the PG from L. monocytogenes demonstrated deacetylation of N-acetylglucosamine residues. We identified a PG N-deacetylase gene, pgdA, in L. monocytogenes genome sequence. Inactivation of pgdA revealed the key role of this PG modification in bacterial virulence because the mutant was extremely sensitive to the bacteriolytic activity of lysozyme, and growth was severely impaired after oral and i.v. inoculations. Within macrophage vacuoles, the mutant was rapidly destroyed and induced a massive IFN-beta response in a TLR2 and Nod1-dependent manner. Together, these results reveal that PG N-deacetylation is a highly efficient mechanism used by Listeria to evade innate host defenses. The presence of deacetylase genes in other pathogenic bacteria indicates that PG N-deacetylation could be a general mechanism used by bacteria to evade the host innate immune system. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/17215377; pasteur-00139188; https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188Test; https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/documentTest; https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/SUPPLEMENTARY_MATERIAL.pdfTest; https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/Listeria_Figures.pdfTest; https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/Listeria_paper-1.pdfTest; https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/Listeria_SupplemFigures.pdfTest; PRODINRA: 250100; PUBMED: 17215377 |
| DOI: | 10.1073/pnas.0609672104 |
| الإتاحة: | https://doi.org/10.1073/pnas.0609672104Test https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188Test https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/documentTest https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/SUPPLEMENTARY_MATERIAL.pdfTest https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/Listeria_Figures.pdfTest https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/Listeria_paper-1.pdfTest https://hal-pasteur.archives-ouvertes.fr/pasteur-00139188/file/Listeria_SupplemFigures.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E6F0EFF2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.0609672104 |
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