دورية أكاديمية
MicroRNA alterations in iPSC-derived dopaminergic neurons from Parkinson disease patients
| العنوان: | MicroRNA alterations in iPSC-derived dopaminergic neurons from Parkinson disease patients |
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| المؤلفون: | Tolosa E., Botta-Orfila T., Morato X., Calatayud C., Ferrer-Lorente R., Marti M. -J., Fernandez M., Gaig C., Raya A., Consiglio A., Ezquerra M., Fernandez-Santiago R. |
| المساهمون: | Tolosa, E., Botta-Orfila, T., Morato, X., Calatayud, C., Ferrer-Lorente, R., Marti, M. -J., Fernandez, M., Gaig, C., Raya, A., Consiglio, A., Ezquerra, M., Fernandez-Santiago, R. |
| سنة النشر: | 2018 |
| المجموعة: | Università degli Studi di Brescia: OPENBS - Open Archive UniBS |
| مصطلحات موضوعية: | Dopaminergic neuron (DAn), Leucine-rich repeat kinase 2 (LRRK2), microRNA (miRNA), Parkinson disease (PD), Transcription factor (TF), Adult, Aged, Cell Differentiation, Dopaminergic Neuron, Down-Regulation, Female, Gene Expression, Gene Expression Profiling, Human, Induced Pluripotent Stem Cell, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2, Male, MicroRNA, Middle Aged, Neural Stem Cell, Parkinson Disease, Up-Regulation |
| الوصف: | MicroRNA (miRNA) misregulation in peripheral blood has been linked to Parkinson disease (PD) but its role in the disease progression remains elusive. We performed an explorative genome-wide study of miRNA expression levels in dopaminergic neurons (DAn) from PD patients generated by somatic cell reprogramming and induced pluripotent stem cells differentiation. We quantified expression levels of 377 miRNAs in DAn from 3 sporadic PD patients (sPD), 3 leucine-rich repeat kinase 2–associated PD patients (L2PD) (total 6 PD), and 4 healthy controls. We identified differential expression of 10 miRNA of which 5 were upregulated in PD (miR-9-5p, miR-135a-5p, miR-135b-5p, miR-449a, and miR-449b-5p) and 5 downregulated (miR-141–3p, miR-199a-5p, miR-299–5p, miR-518e-3p, and miR-519a-3p). Changes were similar in sPD and L2PD. Integrative analysis revealed significant correlations between miRNA/mRNA expression. Moreover, upregulation of miR-9-5p and miR-135b-5p was associated with downregulation of transcription factors related to the DNA hypermethylation of enhancer elements in PD DAn (FOXA1 and NR3C1). In summary, miRNA changes are associated with monogenic L2PD and sPD and co-occur with epigenetic changes in DAn from PD patients. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29935433; info:eu-repo/semantics/altIdentifier/wos/WOS:000439651000029; volume:69; firstpage:283; lastpage:291; numberofpages:9; journal:NEUROBIOLOGY OF AGING; http://hdl.handle.net/11379/540361Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85048717487 |
| DOI: | 10.1016/j.neurobiolaging.2018.05.032 |
| الإتاحة: | https://doi.org/10.1016/j.neurobiolaging.2018.05.032Test http://hdl.handle.net/11379/540361Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.E6961E1B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.neurobiolaging.2018.05.032 |
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