دورية أكاديمية
Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes
| العنوان: | Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes |
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| المؤلفون: | Gutierrez-Rus, Luis I., Gamiz-Arco, Gloria, Gavira Gallardo, J. A., Gaucher, Eric A., Risso, Valeria A., Sánchez-Ruiz, Jose M. |
| المساهمون: | Ministerio de Ciencia e Innovación (España), Human Frontier Science Program, Department of Defense (US), National Institutes of Health (US), National Science Foundation (US) |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2023 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Catalysis, Heme, Peptides, Polypeptide, Catalyst, Chemical reaction, Controlled study, Effective concentration, Enzyme kinetics |
| الوصف: | Enzymes catalyze the chemical reactions of life. For nearly half of known enzymes, catalysis requires the binding of small molecules known as cofactors. Polypeptide-cofactor complexes likely formed at a primordial stage and became starting points for the evolution of many efficient enzymes. Yet, evolution has no foresight so the driver for the primordial complex formation is unknown. Here, we use a resurrected ancestral TIM-barrel protein to identify one potential driver. Heme binding at a flexible region of the ancestral structure yields a peroxidation catalyst with enhanced efficiency when compared to free heme. This enhancement, however, does not arise from protein-mediated promotion of catalysis. Rather, it reflects the protection of bound heme from common degradation processes and a resulting longer lifetime and higher effective concentration for the catalyst. Protection of catalytic cofactors by polypeptides emerges as a general mechanism to enhance catalysis and may have plausibly benefited primordial polypeptide-cofactor associations. ; This work was supported by Human Frontier Science Program grant RGP0041/2017 (J.M.S.R. and E.A.G.), National Science Foundation grant 2032315 (E.A.G.), Department of Defense grant MURI W911NF-16-1-0372 (E.A.G.), National Institutes of Health grant R01AR069137 (E.A.G.), Spanish Ministry of Science and Innovation/ FEDER Funds grant PID2021-124534OB-100 (J.M.S.R.), and grant PID2020-116261GB-I00 (J.A.G.). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1537-1719 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116261GB-I00/ES/APLICACIONES BIOTECNOLOGICAS DE CRISTALES PROTEICOS/; info:eu-repo/grantAgreement/MINECO//PID2021-124534OB-100; Publisher's version; http://dx.doi.org/10.1093/molbev/msad126Test; Sí; Molecular Biology and Evolution 40 (2023); http://hdl.handle.net/10261/346219Test |
| DOI: | 10.1093/molbev/msad126 |
| الإتاحة: | https://doi.org/10.1093/molbev/msad126Test http://hdl.handle.net/10261/346219Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.E6446AE7 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15371719 |
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| DOI: | 10.1093/molbev/msad126 |