دورية أكاديمية
Correlations between serum and CSF pNfH levels in ALS, FTD and controls: a comparison of three analytical approaches.
| العنوان: | Correlations between serum and CSF pNfH levels in ALS, FTD and controls: a comparison of three analytical approaches. |
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| المؤلفون: | Wilke, Carlo, Pujol-Calderón, Fani, Höglund, Kina, Kuhle, Jens, Synofzik, Matthis, Barro, Christian, Stransky, Elke, Blennow, Kaj, Michalak, Zuzanna, Deuschle, Christian, Jeromin, Andreas, Zetterberg, Henrik, Schüle, Rebecca |
| المصدر: | Clinical chemistry and laboratory medicine 57(10), 1556-1564 (2019). doi:10.1515/cclm-2019-0015 |
| بيانات النشر: | De Gruyter |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/610, Adult, Aged, Alzheimer Disease: blood, Alzheimer Disease: cerebrospinal fluid, Amyotrophic Lateral Sclerosis: blood, Amyotrophic Lateral Sclerosis: cerebrospinal fluid, Biomarkers: blood, Clinical Laboratory Techniques: methods, Disease Progression, Female, Frontotemporal Dementia: blood, Frontotemporal Dementia: cerebrospinal fluid, Humans, Huntington Disease: blood, Huntington Disease: cerebrospinal fluid, Intermediate Filaments, Male, Middle Aged, Neurofilament Proteins: analysis, Neurofilament Proteins: blood, Neurofilament Proteins: cerebrospinal fluid, Phosphorylation, Reproducibility of Results, Serum: metabolism |
| جغرافية الموضوع: | DE |
| الوصف: | Background Phosphorylated neurofilament heavy (pNfH), a neuronal cytoskeleton protein, might provide a promising blood biomarker of neuronal damage in neurodegenerative diseases (NDDs). The best analytical approaches to measure pNfH levels and whether serum levels correlate with cerebrospinal fluid (CSF) levels in NDDs remain to be determined. Methods We here compared analytical sensitivity and reliability of three novel analytical approaches (homebrew Simoa, commercial Simoa and ELISA) for quantifying pNfH in both CSF and serum in samples of amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD) and control subjects. Results While all three assays showed highly correlated CSF measurements, Simoa assays also yielded high between-assay correlations for serum measurements (ϱ = 0.95). Serum levels also correlated strongly with CSF levels for Simoa-based measurements (both ϱ = 0.62). All three assays allowed distinguishing ALS from controls by increased CSF pNfH levels, and Simoa assays also by increased serum pNfH levels. pNfH levels were also increased in FTD. Conclusions pNfH concentrations in CSF and, if measured by Simoa assays, in blood might provide a sensitive and reliable biomarker of neuronal damage, with good between-assay correlations. Serum pNfH levels measured by Simoa assays closely reflect CSF levels, rendering serum pNfH an easily accessible blood biomarker of neuronal damage in NDDs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/pmid:31251725; info:eu-repo/semantics/altIdentifier/issn/0939-4974; info:eu-repo/semantics/altIdentifier/issn/1437-4331; info:eu-repo/semantics/altIdentifier/issn/1434-6621; https://pub.dzne.de/record/141491Test; https://pub.dzne.de/search?p=id:%22DZNE-2020-07815%22Test |
| الإتاحة: | https://doi.org/10.1515/cclm-2019-0015Test https://pub.dzne.de/record/141491Test https://pub.dzne.de/search?p=id:%22DZNE-2020-07815%22Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.E630EBD4 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |