دورية أكاديمية
The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
| العنوان: | The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes |
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| المؤلفون: | García García, Laura, Fernández Tabanera, Enrique, Cervera, Saint T, Melero Fernandez de Mera, Raquel Maria, Josa, Santiago, González-González, Laura, Rodríguez-Martín, Carlos, Grünewald, Thomas G P, Alonso, Javier |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Digital Deposit e-UCJC (Universidad Camilo José Cela) |
| مصطلحات موضوعية: | Biología Celular y Molecular, Ciencias Biomédicas, Ewing sarcoma, EWSR1-FLI1, GGAA-microsatellites, FEZF11 |
| الوصف: | Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a spe cific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knock down inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell lines |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2072-6694 |
| العلاقة: | García-García L, Fernández-Tabanera E, Cervera ST, Melero-Fernández de Mera RM, Josa S, González-González L, Rodríguez-Martín C, Grünewald TGP, Alonso J. The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes. Cancers (Basel). 2021 Nov 12;13(22):5668. doi:10.3390/cancers13225668. PMID: 34830820; PMCID: PMC8616448.; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdfTest; http://hdl.handle.net/20.500.12020/1205Test |
| DOI: | 10.3390/CANCERS13225668 |
| الإتاحة: | https://doi.org/20.500.12020/1205Test https://doi.org/10.3390/CANCERS13225668Test https://doi.org/10.3390/cancers13225668Test https://hdl.handle.net/20.500.12020/1205Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdfTest |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; openAccess |
| رقم الانضمام: | edsbas.E56294CF |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20726694 |
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| DOI: | 10.3390/CANCERS13225668 |