دورية أكاديمية
The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells
| العنوان: | The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells |
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| المؤلفون: | Chiaruttini, G., Piperno, G.M., Jouve, M., De Nardi, F., Larghi, P., Peden, A.A., Baj, G., Müller, S., Valitutti, S., Galli, T., Benvenuti, F. |
| بيانات النشر: | Elsevier (Cell Press) |
| سنة النشر: | 2016 |
| المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| الوصف: | Interleukin-12 (IL-12), produced by dendritic cells in response to activation, is central to pathogen eradication and tumor rejection. The trafficking pathways controlling spatial distribution and intracellular transport of IL-12 vesicles to the cell surface are still unknown. Here, we show that intracellular IL-12 localizes in late endocytic vesicles marked by the SNARE VAMP7. Dendritic cells (DCs) from VAMP7-deficient mice are partially impaired in the multidirectional release of IL-12. Upon encounter with antigen-specific T cells, IL-12-containing vesicles rapidly redistribute at the immune synapse and release IL-12 in a process entirely dependent on VAMP7 expression. Consistently, acquisition of effector functions is reduced in T cells stimulated by VAMP7-null DCs. These results provide insights into IL-12 intracellular trafficking pathways and show that VAMP7-mediated release of IL-12 at the immune synapse is a mechanism to transmit innate signals to T cells. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://eprints.whiterose.ac.uk/105416/1/1-s2.0-S2211124716301760-main.pdfTest; Chiaruttini, G., Piperno, G.M., Jouve, M. et al. (8 more authors) (2016) The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells. Cell Reports, 14 (11). pp. 2624-2636. ISSN 2211-1247 |
| DOI: | 10.1016/j.celrep.2016.02.055 |
| الإتاحة: | https://doi.org/10.1016/j.celrep.2016.02.055Test https://eprints.whiterose.ac.uk/105416Test/ https://eprints.whiterose.ac.uk/105416/1/1-s2.0-S2211124716301760-main.pdfTest |
| حقوق: | cc_by_nc_nd_4 |
| رقم الانضمام: | edsbas.E4CD5E76 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2016.02.055 |
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