التفاصيل البيبلوغرافية
| العنوان: |
Cell‐Derived Vesicles as TRPC1 Channel Delivery Systems for the Recovery of Cellular Respiratory and Proliferative Capacities |
| المؤلفون: |
Kurth, Felix, Tai, Yee Kit, Parate, Dinesh, van Oostrum, Marc, Schmid, Yannick R. F., Toh, Shi Jie, Yap, Jasmine Lye Yee, Wollscheid, Bernd, Othman, Alaa, Dittrich, Petra S., Franco‐Obregón, Alfredo |
| المساهمون: |
National University of Singapore, European Research Council, Eidgenössische Technische Hochschule Zürich, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Lee Foundation |
| المصدر: |
Advanced Biosystems ; volume 4, issue 11 ; ISSN 2366-7478 2366-7478 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2020 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Pulsed electromagnetic fields (PEMFs) are capable of specifically activating a TRPC1‐mitochondrial axis underlying cell expansion and mitohormetic survival adaptations. This study characterizes cell‐derived vesicles (CDVs) generated from C2C12 murine myoblasts and shows that they are equipped with the sufficient molecular machinery to confer mitochondrial respiratory capacity and associated proliferative responses upon their fusion with recipient cells. CDVs derived from wild type C2C12 myoblasts include the cation‐permeable transient receptor potential (TRP) channels, TRPC1 and TRPA1, and directly respond to PEMF exposure with TRPC1‐mediated calcium entry. By contrast, CDVs derived from C2C12 muscle cells in which TRPC1 has been genetically knocked‐down using CRISPR/Cas9 genome editing, do not. Wild type C2C12‐derived CDVs are also capable of restoring PEMF‐induced proliferative and mitochondrial activation in two C2C12‐derived TRPC1 knockdown clonal cell lines in accordance to their endogenous degree of TRPC1 suppression. C2C12 wild type CDVs respond to menthol with calcium entry and accumulation, likewise verifying TRPA1 functional gating and further corroborating compartmental integrity. Proteomic and lipidomic analyses confirm the surface membrane origin of the CDVs providing an initial indication of the minimal cellular machinery required to recover mitochondrial function. CDVs hence possess the potential of restoring respiratory and proliferative capacities to senescent cells and tissues. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/adbi.202000146 |
| الإتاحة: |
https://doi.org/10.1002/adbi.202000146Test |
| حقوق: |
http://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: |
edsbas.E463BCC9 |
| قاعدة البيانات: |
BASE |
