التفاصيل البيبلوغرافية
| العنوان: |
IL‐2 abolishes fibroblast proliferation in long‐term bone marrow culture by inhibition of an accessory cell |
| المؤلفون: |
Carron, J. A., Cawley, J. C. |
| المصدر: |
British Journal of Haematology ; volume 79, issue 3, page 377-381 ; ISSN 0007-1048 1365-2141 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
1991 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Addition of interleukin‐2 (IL‐2 (>250 U/ml) during the first 3 d of long‐term bone marrow culture (LTBMC) permanently abolished the fibroblast component of the stromal layer, even when IL‐2 was removed after the 3 d culture period. When IL‐2 was added at more than 72 h after initiation of culture, no effect was observed. Stromal growth in IL‐2‐treated culture was restored by addition of irradiated bone marrow, indicating that the IL‐2 inhibited an accessory cell rather than the fibroblast directly. Accessory cells were shown to be necessary for fibroblast proliferation at low cell densities and were also inhibited by IL‐2. The accessory cell effect could not be replaced by LTBMC supernatants or extracellular matrix. It is suggested that these observations are relevant to the suppression of haemopoiesis observed in patients receiving IL‐2. Addition of interleukin‐2 (IL‐2 (>250 U/ml) during the first 3 d of long‐term bone marrow culture (LTBMC) permanently abolished the fibroblast component of the stromal layer, even when IL‐2 was removed after the 3 d culture period. When IL‐2 was added at more than 72 h after initiation of culture, no effect was observed. Stromal growth in IL‐2‐treated culture was restored by addition of irradiated bone marrow, indicating that the IL‐2 inhibited an accessorycell rather than the fibroblast directly. Accessory cells were shown to be necessary for fibroblast proliferation at low cell densities and were also inhibited by IL‐2. The accessory cell effect could not be replaced by LTBMC supernatants or extracellular matrix. It is suggested that these observations are relevant to the suppression of haemopoiesis observed in patients receiving IL‐2. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/j.1365-2141.1991.tb08044.x |
| الإتاحة: |
https://doi.org/10.1111/j.1365-2141.1991.tb08044.xTest |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.E3DD236C |
| قاعدة البيانات: |
BASE |
