دورية أكاديمية
Localizing Genome Segments and Protein Products of a Multipartite Virus in Host Plant Cells
| العنوان: | Localizing Genome Segments and Protein Products of a Multipartite Virus in Host Plant Cells |
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| المؤلفون: | Vernerey, Marie-Stéphanie, Pirolles, Elodie, Blanc, Stéphane, Sicard, Anne |
| المساهمون: | Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| المصدر: | ISSN: 2331-8325 ; Bio-protocol ; https://hal.inrae.fr/hal-02529712Test ; Bio-protocol , 2019, 9 (23), pp.1-14. ⟨10.21769/BioProtoc.3443⟩. |
| بيانات النشر: | HAL CCSD Bio-protocol LCC |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | FBNSV, Fluorescence microscopy, Nanovirus, Vicia Faba, In situ hybridization (ISH), Immunolocalization, Plant, Virus, Multipartite virus, [SDV]Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | BGPI : équipe 2 ; International audience ; A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. This concept applied to multipartite viruses-which have a genome composed of two or more nucleic acid segments, each individually encapsulated-implies that all segments constituting a viral genome need to coinfect the same host cell for replication to occur. Would this requirement be verified, it would constitute a major cost for extreme cases of multipartition such as the Faba bean necrotic stunt virus (FBNSV, nanovirus) whose genome is composed of eight complementary segments, each encoding a single gene (Grigoras et al., 2009). To address this question, we followed the distribution of the FBNSV genome segments by fluorescence in situ hybridization combined to immunolocalization of the replication-controlling viral protein within the cells of the host plant: Vicia Faba.A rapid and efficient protocol to localize viral transcripts in plant and insect hosts has been developed earlier (Ghanim et al., 2009). We here improve this method by using random-primed labeled probes and apply it to the detection and quantification of the individual segments composing the FBNSV genome. Moreover, we combine this technique with immunolocalization so that both viral segments and proteins can be visualized within the same samples. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02529712; https://hal.inrae.fr/hal-02529712Test; https://hal.inrae.fr/hal-02529712/documentTest; https://hal.inrae.fr/hal-02529712/file/2020_vernerey_bio-protocol.pdfTest; WOS: 000519522300004 |
| DOI: | 10.21769/BioProtoc.3443 |
| الإتاحة: | https://doi.org/10.21769/BioProtoc.3443Test https://hal.inrae.fr/hal-02529712Test https://hal.inrae.fr/hal-02529712/documentTest https://hal.inrae.fr/hal-02529712/file/2020_vernerey_bio-protocol.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E1FB2317 |
| قاعدة البيانات: | BASE |
| DOI: | 10.21769/BioProtoc.3443 |
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