دورية أكاديمية
Targeted knock-in mice expressing the oxidase-fixed form of xanthine oxidoreductase favor tumor growth.
العنوان: | Targeted knock-in mice expressing the oxidase-fixed form of xanthine oxidoreductase favor tumor growth. |
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المؤلفون: | Kusano, T., Ehirchiou, D., Matsumura, T., Chobaz, V., Nasi, S., Castelblanco, M., So, A., Lavanchy, C., Acha-Orbea, H., Nishino, T., Okamoto, K., Busso, N. |
المصدر: | Nature communications, vol. 10, no. 1, pp. 4904 |
سنة النشر: | 2019 |
المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
مصطلحات موضوعية: | Animals, Cell Proliferation, Female, Gene Knock-In Techniques, Humans, Macrophages/enzymology, Macrophages/metabolism, Male, Mice, Inbred C57BL, Neoplasms/enzymology, Neoplasms/genetics, Neoplasms/metabolism, Neoplasms/physiopathology, Reactive Oxygen Species/metabolism, Xanthine Dehydrogenase/genetics, Xanthine Dehydrogenase/metabolism, Xanthine Oxidase/genetics, Xanthine Oxidase/metabolism |
الوصف: | Xanthine oxidoreductase has been implicated in cancer. Nonetheless, the role played by its two convertible forms, xanthine dehydrogenase (XDH) and oxidase (XO) during tumorigenesis is not understood. Here we produce XDH-stable and XO-locked knock-in (ki) mice to address this question. After tumor transfer, XO ki mice show strongly increased tumor growth compared to wild type (WT) and XDH ki mice. Hematopoietic XO expression is responsible for this effect. After macrophage depletion, tumor growth is reduced. Adoptive transfer of XO-ki macrophages in WT mice increases tumor growth. In vitro, XO ki macrophages produce higher levels of reactive oxygen species (ROS) responsible for the increased Tregs observed in the tumors. Blocking ROS in vivo slows down tumor growth. Collectively, these results indicate that the balance of XO/XDH plays an important role in immune surveillance of tumor development. Strategies that inhibit the XO form specifically may be valuable in controlling cancer growth. |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 2041-1723 |
العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31659168; info:eu-repo/semantics/altIdentifier/eissn/2041-1723; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_AB744ACBDD116; https://serval.unil.ch/notice/serval:BIB_AB744ACBDD11Test; urn:issn:2041-1723; https://serval.unil.ch/resource/serval:BIB_AB744ACBDD11.P001/REF.pdfTest; http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_AB744ACBDD116Test |
DOI: | 10.1038/s41467-019-12565-z |
الإتاحة: | https://doi.org/10.1038/s41467-019-12565-zTest https://serval.unil.ch/notice/serval:BIB_AB744ACBDD11Test https://serval.unil.ch/resource/serval:BIB_AB744ACBDD11.P001/REF.pdfTest http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_AB744ACBDD116Test |
حقوق: | info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0Test/ |
رقم الانضمام: | edsbas.E1F33E43 |
قاعدة البيانات: | BASE |
تدمد: | 20411723 |
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DOI: | 10.1038/s41467-019-12565-z |