دورية أكاديمية
CLDN15 is a novel diagnostic marker for malignant pleural mesothelioma
| العنوان: | CLDN15 is a novel diagnostic marker for malignant pleural mesothelioma |
|---|---|
| المؤلفون: | Watanabe, Masayuki, Higashi, Tomohito, Ozeki, Kana, Higashi, Atsuko Y., Sugimoto, Kotaro, Mine, Hayato, Takagi, Hironori, Ozaki, Yuki, Muto, Satoshi, Okabe, Naoyuki, Matsumura, Yuki, Hasegawa, Takeo, Shio, Yutaka, Suzuki, Hiroyuki, Chiba, Hideki |
| المساهمون: | Japan Society for the Promotion of Science |
| المصدر: | Scientific Reports ; volume 11, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | Malignant mesothelioma is a cancer with a poor survival rate. It is difficult to diagnose mesotheliomas because they show a variety of histological patterns similar to those of various other cancers. However, since currently used positive markers for mesotheliomas may show false positives or false negatives, a novel mesothelial positive marker is required. In the present study, we screened 25 claudins and found that claudin-15 is expressed in the mesothelial cells. We made new rat anti-human claudin-15 (CLDN15) monoclonal antibodies that selectively recognize CLDN15, and investigated whether CLDN15 is a good positive marker for malignant pleural mesotheliomas (MPMs) using MPM tissue samples by immunohistochemistry and semi-quantification of the expression level using an immunoreactive score (IRS) method. Of 42 MPM samples, 83% were positive for CLDN15. The positive ratio was equal to or greater than other positive markers for MPMs including calretinin (81%), WT-1 (50%), and D2-40 (81%). In 50 lung adenocarcinoma sections, four cases were positive for CLDN15 and the specificity (92%) was comparable with other markers (90–100%). Notably, CLDN15 was rarely detected in 24 non-mesothelial tumors in the tissue microarray (12/327 cases). In conclusion, CLDN15 can be used in the clinical setting as a positive marker for MPM diagnosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-021-91464-0 |
| الإتاحة: | https://doi.org/10.1038/s41598-021-91464-0Test https://www.nature.com/articles/s41598-021-91464-0.pdfTest https://www.nature.com/articles/s41598-021-91464-0Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.E14337CA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-021-91464-0 |
|---|