دورية أكاديمية
Lcc1 and Lcc5 are the main laccases secreted in liquid cultures of Coprinopsis cinerea strains
| العنوان: | Lcc1 and Lcc5 are the main laccases secreted in liquid cultures of Coprinopsis cinerea strains |
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| المؤلفون: | Ruehl, Martin, Majcherczyk, Andrzej, Kuees, Ursula |
| بيانات النشر: | Springer |
| سنة النشر: | 2013 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | The litter-degrading dung fungus Coprinopsis cinerea has the high number of seventeen different laccase genes. In this work, ten different monokaryons were compared in their ability to produce laccases in two different complete media at different temperatures. Few strains showed laccase activity at the optimal growth temperature of 37 degrees C. Nine of the strains gave laccase activities between 0.2 and 5.9 U mL(-1) at the suboptimal temperature of 25 degrees C in mKjalke medium. Laccase activities in YMG/T medium were detected for only three strains (0.5-4.5 U mL(-1)). Zymograms of supernatants from mKjalke medium resulted in total in 10 different laccase bands but strains differed in distribution. LC-MS/MS analysis with Mascot searches of the annotated C. cinerea genome identified isoenzymes from five different genes (Lcc1, Lcc2, Lcc5, Lcc9 and Lcc10) and of Lcc1 three and of Lcc5 two distinct electrophoretical forms. Lcc1 and Lcc5 were expressed in all laccase positive strains, but not all forms were found in all of the strains. Lcc2, Lcc9 and Lcc10 occurred only in three strains as minor laccases, indicating that Lcc1 and Lcc5 are the main laccases of C. cinerea secreted in liquid mKjalke medium. ; Ministry of Science and Culture of Lower Saxony [ZN 2043] |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0003-6072 |
| العلاقة: | https://resolver.sub.uni-goettingen.de/purl?gro-2/29973Test; 000319268600010; https://resolver.sub.uni-goettingen.de/purl?gs-1/10338Test |
| DOI: | 10.1007/s10482-013-9883-7 |
| الإتاحة: | https://doi.org/10.1007/s10482-013-9883-7Test https://resolver.sub.uni-goettingen.de/purl?gro-2/29973Test https://resolver.sub.uni-goettingen.de/purl?gs-1/10338Test |
| حقوق: | Goescholar ; https://goescholar.uni-goettingen.de/licensesTest |
| رقم الانضمام: | edsbas.E0DA1548 |
| قاعدة البيانات: | BASE |
| تدمد: | 00036072 |
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| DOI: | 10.1007/s10482-013-9883-7 |