التفاصيل البيبلوغرافية
| العنوان: |
IN VITRO STIMULATION OF LYMPHOCYTES BY NEUTRAL PROTEINASES FROM HUMAN POLYMORPHONUCLEAR LEUKOCYTE GRANULES |
| المؤلفون: |
T. L. Vischer, U. Bretz, M. Baggiolini |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/29/2b/J_Exp_Med_1976_Oct_1_144(4)_863-872.tar.gz |
| المجموعة: |
CiteSeerX |
| الوصف: |
Human polymorphonuclear leukocytes (PMNP contain considerable amounts of neutral proteinases in their azurophil granules (1). Upon phagocytosis, these enzymes are released from the granules into phagocytic vacuoles and into the extracellular medium (2) where they may damage a large variety of tissue components (3). Neutral proteinases such as trypsin, chymotrypsin, Pronase, and pancreatic elastase are known to induce lymphocyte transformation and proliferation (4-7). In chronic inflammatory processes, proteinases which are released from PMN may act on resident lymphocytes and modulate their behavior. In the present work, we have investigated the effects of purified elastase and cathepsin G 2 from human PMN on lymphocytes of different sources. Materials and Methods Isolation of Neutral Proteinases from Human PMN. PMN from pooled buffy coats of donor blood were purified to about 90%, as estimated by differential counts on Giemsa-stained smears. The cells were homogenized in 0.34 M sucrose (10), and the granules collected by centrifuging the |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.285.2388Test |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.E00E44A7 |
| قاعدة البيانات: |
BASE |
