دورية أكاديمية
Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle
| العنوان: | Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle |
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| المؤلفون: | Iyer, Shama R, Hsia, Ru-Ching, Folker, Eric S, Lovering, Richard M |
| المصدر: | 150 ; 111338 ; England |
| بيانات النشر: | Elsevier Inc. |
| سنة النشر: | 2021 |
| المجموعة: | UMB Digital Archive (University of Maryland, Baltimore) |
| مصطلحات موضوعية: | Biology of aging, Mice, Muscles, Sarcopenia |
| الوصف: | Mechanical forces are conducted through myofibers and into nuclei to regulate muscle development, hypertrophy, and homeostasis. We hypothesized that nuclei in aged muscle have changes in the nuclear envelope and associated proteins, resulting in altered markers of mechano-signaling. Methods: YAP/TAZ protein expression and gene expression of downstream targets, Ankrd1 and Cyr61, were evaluated as mechanotransduction indicators. Expression of proteins in the nuclear lamina and the nuclear pore complex (NPC) were assessed, and nuclear morphology was characterized by electron microscopy. Nuclear envelope permeability was assessed by uptake of 70 kDa fluorescent dextran. Results: Nuclear changes with aging included a relative decrease of lamin β1 and Nup107, and a relative increase in Nup93, which could underlie the aberrant nuclear morphology, increased nuclear leakiness, and elevated YAP/TAZ signaling. Conclusion: Aged muscles have hyperactive nuclear-cytoplasmic signaling, indicative of altered nuclear mechanotransduction. These data highlight a possible role for the nucleus in aging-related aberrant mechano-sensing. © 2021 The Authors ; https://doi.org/10.1016/j.exger.2021.111338Test |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Experimental Gerontology; http://hdl.handle.net/10713/15495Test |
| DOI: | 10.1016/j.exger.2021.111338 |
| الإتاحة: | https://doi.org/10.1016/j.exger.2021.111338Test http://hdl.handle.net/10713/15495Test |
| حقوق: | Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved. |
| رقم الانضمام: | edsbas.DF8B200D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.exger.2021.111338 |
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