دورية أكاديمية
Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17
| العنوان: | Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17 |
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| المؤلفون: | Srivastava, Yogesh, Tan, Daisylyn Senna, Malik, Vikas, Weng, Mingxi, Javed, Asif, Cojocaru, Vlad, Wu, Guangming, Veerapandian, Veeramohan, Cheung, Lydia W T, Jauch, Ralf |
| المصدر: | Srivastava , Y , Tan , D S , Malik , V , Weng , M , Javed , A , Cojocaru , V , Wu , G , Veerapandian , V , Cheung , L W T & Jauch , R 2020 , ' Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17 ' , FEBS Journal , vol. 287 , no. 1 , pp. 122-144 . https://doi.org/10.1111/febs.15076Test |
| سنة النشر: | 2020 |
| المجموعة: | KNAW: Research Explorer (Koninklijke Nederlandse Akademie van Wetenschappen / Royal Netherlands Academy of Arts and Sciences) |
| مصطلحات موضوعية: | Animals, Cell Differentiation, Cells, Cultured, Cellular Reprogramming, Embryonic Stem Cells/cytology, Gene Expression Profiling, Humans, Induced Pluripotent Stem Cells/cytology, Mice, Mutation, Missense, Neoplasms/genetics, Octamer Transcription Factor-3/genetics, SOXB1 Transcription Factors/genetics, SOXF Transcription Factors/genetics |
| الوصف: | The functional consequences of cancer-associated missense mutations are unclear for the majority of proteins. We have previously demonstrated that the activity of SOX and Pit-Oct-Unc (POU) family factors during pluripotency reprogramming can be switched and enhanced with rationally placed point mutations. Here, we interrogated cancer mutation databases and identified recurrently mutated positions at critical structural interfaces of the DNA-binding domains of paralogous SOX and POU family transcription factors. Using the conversion of mouse embryonic fibroblasts to induced pluripotent stem cells as functional readout, we identified several gain-of-function mutations that enhance pluripotency reprogramming by SOX2 and OCT4. Wild-type SOX17 cannot support reprogramming but the recurrent missense mutation SOX17-V118M is capable of inducing pluripotency. Furthermore, SOX17-V118M promotes oncogenic transformation, enhances thermostability and elevates cellular protein levels of SOX17. We conclude that the mutational profile of SOX and POU family factors in cancer can guide the design of high-performance reprogramming factors. Furthermore, we propose cellular reprogramming as a suitable assay to study the functional impact of cancer-associated mutations. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://pure.knaw.nl/portal/en/publications/71f1888a-7614-4101-8c39-09d156b776c5Test |
| DOI: | 10.1111/febs.15076 |
| الإتاحة: | https://doi.org/10.1111/febs.15076Test https://doi.org/20.500.11755/71f1888a-7614-4101-8c39-09d156b776c5Test https://pure.knaw.nl/portal/en/publications/71f1888a-7614-4101-8c39-09d156b776c5Test https://hdl.handle.net/20.500.11755/71f1888a-7614-4101-8c39-09d156b776c5Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.DEE43092 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/febs.15076 |
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