كتاب
Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants
| العنوان: | Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants |
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| المؤلفون: | Jupe, Florian, Chen, Xinwei, Verweij, Walter, Witek, Kamel, Jones, Jonathan D.G., Hein, Ingo |
| المساهمون: | Birch, Paul, Jones, John T., Bos, Jorunn I.B. |
| المصدر: | Jupe , F , Chen , X , Verweij , W , Witek , K , Jones , J D G & Hein , I 2014 , Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants . in P Birch , J T Jones & J I B Bos (eds) , Plant-Pathogen Interactions . vol. 1127 , Methods in Molecular Biology , Springer , pp. 291-303 . https://doi.org/10.1007/978-1-62703-986-4_22Test |
| بيانات النشر: | Springer |
| سنة النشر: | 2014 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | Agilent SureSelect, High-throughput sequencing, NB-LRR genes, Target enrichment, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1311, name=Genetics |
| الوصف: | Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher readdepth for selected genes and is therefore suitable for determination of sequence polymorphisms and enables multiplexing of samples. Target enrichment also provides a means to annotate specific gene families within the sequenced organisms without the requirements for gene models. Here we describe enrichment procedures for NB-LRR-type plant resistance genes that can, for example, be used to establish the NB-LRR gene complements of individual plants and to map resistances more rapidly using a bulked segregant analysis. |
| نوع الوثيقة: | book part |
| اللغة: | English |
| ردمك: | 978-1-62703-985-7 1-62703-985-6 |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/2df6bac5-7917-4c81-bf04-52c327bb6f5dTest; urn:ISBN:978-1-62703-985-7 |
| DOI: | 10.1007/978-1-62703-986-4_22 |
| الإتاحة: | https://doi.org/10.1007/978-1-62703-986-4_22Test https://discovery.dundee.ac.uk/en/publications/2df6bac5-7917-4c81-bf04-52c327bb6f5dTest http://www.scopus.com/inward/record.url?scp=84908475953&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.DE7E58A3 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781627039857 1627039856 |
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| DOI: | 10.1007/978-1-62703-986-4_22 |