التفاصيل البيبلوغرافية
| العنوان: |
Extracellular matrix remodeling following myocardial infarction influences the therapeutic potential of mesenchymal stem cells |
| المؤلفون: |
Sullivan, Kelly, Quinn, Kyle, Tang, Katherine, Georgakoudi, Irene, Black, Lauren |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2014 |
| المجموعة: |
BioMed Central |
| الوصف: |
Introduction Although stem cell therapy is a promising treatment for myocardial infarction, the minimal functional improvements observed clinically limit its widespread application. A need exists to maximize the therapeutic potential of these stem cells by first understanding what factors within the infarct microenvironment affect their ability to regenerate the necrotic tissue. In this study, we assessed both differentiation capacity and paracrine signaling as a function of extracellular matrix remodeling after myocardial infarction. Methods Mechanical and compositional changes to the decellularized infarcted myocardium were characterized to understand how the extracellular environment, specifically, was altered as a function of time after coronary artery ligation in Sprague–Dawley rats. These alterations were first modeled in a polyacrylamide gel system to understand how the variables of composition and stiffness drive mesenchymal stem cell differentiation towards a cardiac lineage. Finally, the paracrine secretome was characterized as a function of matrix remodeling through gene and protein expression and conditioned media studies. Results The decellularized infarct tissue revealed significant alterations in both the mechanical and compositional properties of the ECM with remodeling following infarction. This altered microenvironment dynamically regulates the potential for early cardiac differentiation. Whereas Nkx2.5 expression is limited in the presence of chronic remodeled matrix of increased stiffness, GATA4 expression is enhanced. In addition, the remodeled matrix promotes the expression of several proangiogenic, prosurvival, antifibrotic, and immunomodulatory growth factors. In particular, an increase in HGF and SDF1 expression and secretion by mesenchymal stem cells can rescue oxidatively stressed cardiomyocytes in vitro . Conclusions This study demonstrated that decellularization of diseased tissue allows for the exclusive analysis of the remodeled matrix and its ability to influence ... |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://stemcellres.com/content/5/1/14Test |
| الإتاحة: |
http://stemcellres.com/content/5/1/14Test |
| حقوق: |
Copyright 2014 Sullivan et al.; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.DE507EA6 |
| قاعدة البيانات: |
BASE |
