دورية أكاديمية
أعرض في EDS

Gestational age-related patterns of AMOT methylation are revealed in preterm infant endothelial progenitors.

التفاصيل البيبلوغرافية
العنوان: Gestational age-related patterns of AMOT methylation are revealed in preterm infant endothelial progenitors.
المؤلفون: Vinci, G., Buffat, C., Simoncini, S., Boubred, F., Ligi, I., Dumont, F., Le Bonniec, B., Fournier, T., Vaiman, D., Dignat-George, F., Simeoni, U.
المصدر: PloS one, vol. 12, no. 10, pp. e0186321
سنة النشر: 2017
المجموعة: Université de Lausanne (UNIL): Serval - Serveur académique lausannois
مصطلحات موضوعية: Adult, CpG Islands, DNA Methylation, Endothelial Progenitor Cells/metabolism, Epigenesis, Genetic, Female, Fetal Blood/metabolism, Humans, Infant, Premature/growth & development, Premature/metabolism, Small for Gestational Age/growth & development, Small for Gestational Age/metabolism, Intercellular Signaling Peptides and Proteins/genetics, Intercellular Signaling Peptides and Proteins/metabolism, Male, Maternal Age, Membrane Proteins/genetics, Membrane Proteins/metabolism, Promoter Regions, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA
الوصف: Preterm birth is associated with altered angiogenesis and with increased risk of cardiovascular dysfunction and hypertension at adulthood. We previously demonstrated that in preterm newborns circulating cord blood endothelial progenitor cells (ECFC), responsible for angio/vasculogenesis, are reduced in number and display altered angiogenic properties. Altered angiogenic function was associated with a decreased expression of pro-angiogenic genes, among which the AMOT gene which is a strong positive regulator of angiogenesis. Such dysregulation may be related to epigenetic factors. In this study we analyse the methylation profiling of the AMOT gene during development, through a comparative analysis of the cord blood ECFC of preterm newborns and their term counterpart. We used both cloning-sequencing and pyrosequencing experiments to perform a comparative analysis of the DNA methylation profile of the promoter CpG island of AMOT gene in the cord blood ECFC of 16 preterm newborns (28-35 weeks gestational age-GA) and 15 term newborns (>37 weeks GA). Twenty nine clones (obtained from 2 term newborns) and forty clones (obtained from 3 preterm newborns) were sequenced. The AMOT gene methylation rate was significantly higher in preterm compared to term newborns (4.5% versus 2.5% respectively: χ2 = 3.84; P = 1.8 10-02). Bisulfite pyrosequencing identified four CpG dinucleotides with significantly higher methylation levels in preterm newborns. This CpG-targeted methylation significantly decreased with increasing gestational age. These findings highlight importance of pro-angiogenic AMOT gene methylation in ECFC, suggesting that epigenetic mechanisms may control the regulation of angiogenesis during development. Therefore they pave the way to specific short term and long term complications of preterm birth by altered angiogenesis.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 1932-6203
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/29036193; info:eu-repo/semantics/altIdentifier/eissn/1932-6203; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_F79E5DFB6FE85; https://serval.unil.ch/notice/serval:BIB_F79E5DFB6FE8Test; urn:issn:1932-6203; https://serval.unil.ch/resource/serval:BIB_F79E5DFB6FE8.P001/REF.pdfTest; http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_F79E5DFB6FE85Test
DOI: 10.1371/journal.pone.0186321
الإتاحة: https://doi.org/10.1371/journal.pone.0186321Test
https://serval.unil.ch/notice/serval:BIB_F79E5DFB6FE8Test
https://serval.unil.ch/resource/serval:BIB_F79E5DFB6FE8.P001/REF.pdfTest
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_F79E5DFB6FE85Test
حقوق: info:eu-repo/semantics/openAccess ; Copying allowed only for non-profit organizations ; https://serval.unil.ch/disclaimerTest
رقم الانضمام: edsbas.DD6DC8FD
قاعدة البيانات: BASE
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0186321