التفاصيل البيبلوغرافية
| العنوان: |
Improving home haemodialysis: Stability evaluation of routine clinical chemistry analytes in blood samples of haemodialysis patients |
| المؤلفون: |
Nonkes, Lourens J.P., Van Gelder, Maaike K., Kemperman, Hans, Abrahams, Alferso C., Boereboom, Frans T.J., ten Berg, Maarten J., Gerritsen, Karin G.F. |
| المساهمون: |
CDL Klinisch Chemici in opleiding, UMC Utrecht, Nefro Vasculaire Geneeskunde, CDL Staf Patiëntenzorg KC, Other research (not in main researchprogram), MS Nefrologie, Circulatory Health, Infection & Immunity, Regenerative Medicine and Stem Cells |
| سنة النشر: |
2019 |
| مصطلحات موضوعية: |
Bicarbonates, Blood specimen collection, Clinical chemistry tests, Haemodialysis, Blood Preservation, Humans, Lactic Acid/blood, Clinical Chemistry Tests/methods, Bicarbonates/blood, Blood Glucose/analysis, Potassium/blood, Hemodialysis, Home/methods, Calcium/blood, Biochemistry, medical, Clinical Biochemistry, Journal Article, Evaluation Studies |
| الوصف: |
Introduction: A growing number of dialysis patients is treated with home haemodialysis. Our current pre-analytical protocols require patients to centrifuge the blood sample and transfer the plasma into a new tube at home. This procedure is prone to errors and precludes accurate bicarbonate measurement, required for determining dialysate bicarbonate concentration and maintaining acid-base status. We therefore evaluated whether cooled overnight storage of gel separated plasma is an acceptable alternative. Materials and methods: Venous blood of 34 haemodialysis patients was collected in 2 lithium heparin blood collection tubes with gel separator (LH PSTTM II, REF 367374; Becton Dickinson, New Jersey, USA). One tube was analysed directly for measurement of bicarbonate, potassium, calcium, phosphate, glucose, urea, lactate, aspartate aminotransferase (AST), and lactate dehydrogenase (LD); whereas the other was centrifuged and stored unopened at 4 °C and analysed 24 h later. To measure analyte stability after 24 h of storage, the mean difference was calculated and compared to the total allowable error (TEa) which was used as acceptance limit. Results: Potassium (Z = - 4.28, P < 0.001), phosphate (Z = - 3.26, P = 0.001), lactate (Z = - 5.11, P < 0.001) and AST (Z = - 2.71, P = 0.007) concentrations were higher, whereas glucose (Z = 4.00, P < 0.001) and LD (Z = 3.13, P = 0.002) showed a reduction. All mean differences were smaller than the TEa and thus not clinically relevant. Bicarbonate (Z = 0.69, P = 0.491), calcium (Z = - 0.23, P = 0.815) and urea (Z = 0.81, P =0.415) concentrations were stable. Conclusions: Our less complex, user-friendly pre-analytical procedure resulted in at least 24 h stability of analytes relevant for monitoring haemodialysis, including bicarbonate. This allows shipment and analysis the next day. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
image/pdf |
| اللغة: |
English |
| تدمد: |
1330-0962 |
| العلاقة: |
https://dspace.library.uu.nl/handle/1874/391370Test |
| الإتاحة: |
https://dspace.library.uu.nl/handle/1874/391370Test |
| حقوق: |
info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: |
edsbas.DCE77150 |
| قاعدة البيانات: |
BASE |
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